In:
American Journal of Veterinary Research, American Veterinary Medical Association (AVMA), Vol. 64, No. 6 ( 2003-06-01), p. 779-784
Abstract:
Objective —To compare growth characteristics of
strains of equine arteritis virus (EAV) of differing virulence to horses in rabbit kidney (RK)-13 cells and
equine endothelial cells (EECs) cultured from the pulmonary artery of a foal. Sample Population —13 strains of EAV, including 11
field isolates of differing virulence to horses; the highly virulent, horse-adapted Bucyrus strain; and the
modified-live virus (MLV) vaccine derived from it. Procedure —The growth characteristics of the 13
strains were compared in EECs and RK-13 cells. Viral nucleoprotein expression, cytopathogenicity, and
plaque size were compared to determine whether growth characteristics of the 13 strains were predictive
of their virulence to horses. Results —Cytopathogenicity, viral nucleoprotein
expression, and plaque size induced by all 13 viruses were similar in RK-13 cells, whereas virulent strains of
EAV caused significantly larger plaques in EECs than did the avirulent strains of EAV. Paradoxically, the
highly attenuated MLV vaccine and 1 field isolate of EAV caused plaques in EECs that were larger than
those caused by any of the other viruses, and sequence analysis confirmed the field isolate of EAV
to be indistinguishable from the MLV vaccine. Conclusions and Clinical Relevance —With the
notable exception of the MLV vaccine, growth of the various strains of EAV in EECs was predictive of their
individual virulence to horses. Thus, EECs provide a relevant and useful model to further characterize
determinants of virulence and attenuation amongst strains of EAV. ( Am J Vet Res 2003;64:779–784)
Type of Medium:
Online Resource
ISSN:
0002-9645
DOI:
10.2460/ajvr.2003.64.779
Language:
Unknown
Publisher:
American Veterinary Medical Association (AVMA)
Publication Date:
2003
detail.hit.zdb_id:
2056942-7
SSG:
22
Permalink