In:
Environmental Microbiology, Wiley, Vol. 18, No. 5 ( 2016-05), p. 1326-1337
Abstract:
To assess the impact of sanitation of a living environment on gut microbiota and development of the immune system, we raised BALB /c mice under three distinct environmental conditions: a specific pathogen‐free animal room ( SPF ), a general animal room ( XZ ) and a farmhouse ( JD ). All other variables like diet, age, genetic background, physiological status and original gut microbiota were controlled for in the three groups. Using high‐throughput sequencing of the 16S rRNA gene, we found that each mouse group had a specific structure of the gut microbial community. Groups JD and XZ harboured a significantly more diverse and richer gut microbiota than did group SPF . Bacteroidetes were significantly more abundant in groups XZ and JD than in group SPF , whereas F irmicutes showed the inverse pattern. Total serum immunoglobulin E ( IgE ) levels were significantly lower in groups XZ and JD than in group SPF . There were no significant differences in gut microbiota diversity and serum IgE concentration between groups JD and XZ , but we found higher abundance of dominant genera in the gut microflora of group JD . We conclude that exposure to soil, house dust and decaying plant material enhances gut microbial diversity and innate immunity. Our results seem to provide new evidence supporting the hygiene hypothesis.
Type of Medium:
Online Resource
ISSN:
1462-2912
,
1462-2920
DOI:
10.1111/emi.2016.18.issue-5
DOI:
10.1111/1462-2920.12895
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2020213-1
SSG:
12
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