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  • 1
    Online Resource
    Online Resource
    Frontiers Media SA ; 2022
    In:  Frontiers in Genetics Vol. 13 ( 2022-6-21)
    In: Frontiers in Genetics, Frontiers Media SA, Vol. 13 ( 2022-6-21)
    Abstract: Anti-inflammatory, antiviral, and anti-cancer treatments are potential applications of nanomaterials in biology. To explore the latest discoveries in nanotechnology, we reviewed the published literature, focusing on co-assembled nanoparticles for anti-inflammatory and anti-tumor properties, and their applications in animal husbandry. The results show that nanoparticles have significant anti-inflammation and anti-tumor effects, demonstrating broad application prospects in animal breeding. Furthermore, pooled evidence suggests that the mechanism is to have a positive impact on inflammation and tumors through the specific drug loading by indirectly or directly targeting the disease sites. Because the precise regulatory mechanism remains unclear, most studies have focused on regulating particular sites or even specific genes in the nucleus by targeting functional co-assembled nanoparticles. Hence, despite the intriguing scenarios for nanotechnology in farmed animals, most results cannot yet be translated into field applications. Overall, nanomaterials outperformed similar materials in terms of anti-inflammatory and anti-tumor. Nanotechnology also has promising applications in animal husbandry and veterinary care, and its application and development in animal husbandry remain an exciting area of research.
    Type of Medium: Online Resource
    ISSN: 1664-8021
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2606823-0
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  • 2
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2022
    In:  Journal of Orthopaedics and Traumatology Vol. 23, No. 1 ( 2022-12)
    In: Journal of Orthopaedics and Traumatology, Springer Science and Business Media LLC, Vol. 23, No. 1 ( 2022-12)
    Abstract: Comminuted fractures of the coronoid process significantly compromise the stability and function of the elbow joint. Reconstruction of the coronoid process with a prosthesis has been suggested as an alternative to restore the architecture. The purpose of this study was to investigate the strength and stability of various methods for the fixation of a coronoid prosthesis by finite element analysis. Materials and methods A coronoid prosthesis was designed based on the morphological information from computed tomography images acquired from 64 subjects in whom the top 40% of the coronoid process height was replaced. Four methods for the fixation of the prosthesis were suggested: (1) a double 2.0-mm fixation bolt, anterior to posterior; (2) a double 2.5-mm fixation bolt, anterior to posterior; (3) a single 4.0-mm fixation bolt, posterior to anterior; (4) a single 4.5-mm fixation bolt, posterior to anterior. The integrated prosthesis-bone constructs were analyzed via the finite element analysis of 10 simulated proximal ulna models with loading applied along the axis of the humerus and with three different elbow flexion angles (30°, 90°, and 130°). The maximum principal stress and the total deformation were quantified and compared. Results A coronoid prosthesis was developed. The maximum principal stress of the fixation bolts occurred around the neck of the fixation bolt. For a comparison of the strengths of the four fixation methods, the maximum principal stress was the lowest for fixation using a single 4.5-mm fixation bolt. The value of the maximum principal stress significantly decreased with increased elbow flexion angle for all fixation methods. The maximum deformation of the fixation bolts occurred at the head of the fixation bolt. For a comparison of the maximum deformations in the four fixation methods, the maximum deformation was the lowest for fixation using a single 4.5-mm fixation bolt. The value of the maximum deformation significantly decreased with increased elbow flexion angle for all fixation methods. Conclusions The present study suggested that fixation of a coronoid prosthesis with a single 4.5-mm fixation bolt from posterior to anterior is an excellent option in terms of the strength and stability. Level of Evidence Experimental study.
    Type of Medium: Online Resource
    ISSN: 1590-9921 , 1590-9999
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2034945-2
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  • 3
    In: British Journal of Pharmacology, Wiley, Vol. 179, No. 12 ( 2022-06), p. 3078-3095
    Abstract: Tacrolimus a first‐line medication used after transplantation can induce β‐cell dysfunction, causing new‐onset diabetes mellitus (NODM). Regenerating islet‐derived protein 3 gamma (Reg3g), a member of the pancreatic regenerative gene family, has been reported to improve type 1 diabetes by promoting β‐cell regeneration. We aim to investigate the role of Reg3g in reversing tacrolimus‐induced β‐cell dysfunction and NODM in mice. Experimental Approach Circulating REG3A (the human homologue of mouse Reg3g) in heart transplantation patients treated with tacrolimus was detected. The glucose‐stimulated insulin secretion and mitochondrial functions, including mitochondria membrane potential (MMP), mitochondria calcium, ATP production, oxygen consumption rate and mitochondrial morphology were investigated in β‐cells. Additionally, effects of Reg3g on tacrolimus‐induced NODM in mice were analysed. Key Results Circulating REG3A level in heart transplantation patients with NODM significantly decreased compared with those without diabetes. Tacrolimus down‐regulated Reg3g via inhibiting STAT3‐mediated transcription activation. Moreover, Reg3g restored glucose‐stimulated insulin secretion suppressed by tacrolimus in β‐cells by improving mitochondrial functions, including increased MMP, mitochondria calcium uptake, ATP production, oxygen consumption rate and contributing to an intact mitochondrial morphology. Mechanistically, Reg3g increased accumulation of pSTAT3(Ser727) in mitochondria by activating ERK1/2‐STAT3 signalling pathway, leading to restoration of tacrolimus‐induced mitochondrial impairment. Reg3g overexpression also effectively mitigated tacrolimus‐induced NODM in mice. Conclusion and Implications Reg3g can significantly ameliorate tacrolimus‐induced β‐cell dysfunction by restoring mitochondrial function in a pSTAT3(Ser727)‐dependent manner. Our observations identify a novel Reg3g‐mediated mechanism that is involved in tacrolimus‐induced NODM and establish the novel role of Reg3g in reversing tacrolimus‐induced β‐cell dysfunction.
    Type of Medium: Online Resource
    ISSN: 0007-1188 , 1476-5381
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2029728-2
    SSG: 15,3
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  • 4
    Online Resource
    Online Resource
    S. Karger AG ; 2018
    In:  Cellular Physiology and Biochemistry Vol. 50, No. 1 ( 2018), p. 246-260
    In: Cellular Physiology and Biochemistry, S. Karger AG, Vol. 50, No. 1 ( 2018), p. 246-260
    Abstract: Background/Aims: Age-related cataract (ARC) remains the leading cause of visual impairment among the elderly population. Long non-coding RNAs (lncRNAs) have emerged as potential regulators in many ocular diseases. However, the role of lncRNAs in nuclear ARC, a subtype of ARC, requires further elucidation. Methods: LncRNA sequencing was performed to identify differentially expressed lncRNAs between the capsules of transparent and nuclear ARC lenses. Expression validation was confirmed by qRT-PCR. MTT assay, Calcein-AM and propidium iodide double staining, Rhodamine 123 and Hoechst double staining, EdU and transwell assay were used to determine the role of H19 or miR-675 in the viability, apoptosis, proliferation and migration of primary cultured human lens epithelial cells (HLECs). Bioinformatics and luciferase reporter assays were used to identify the binding target of miR-675. Results: Sixty-three lncRNAs are differentially expressed between the capsules of transparent and nuclear ARC lenses. One top abundantly expressed lncRNA, H19, is significantly up-regulated in the nuclear ARC lens capsules and positively associated with nuclear ARC grade. H19 knockdown accelerates apoptosis development and reduces the proliferation and migration of HLECs upon oxidative stress. H19 is the precursor of miR-675, and a reduction of H19 inhibits miR-675 expression. miR-675 regulates CRYAA expression by targeting the binding site within the 3’UTR. Moreover, miR-675 increases the proliferation and migration while decreasing the apoptosis of HLECs upon oxidative stress. Conclusion: H19 regulates HLECs function through miR-675-mediated CRYAA expression. This finding would provide a novel insight into the pathogenesis of nuclear ARC.
    Type of Medium: Online Resource
    ISSN: 1015-8987 , 1421-9778
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2018
    detail.hit.zdb_id: 1482056-0
    SSG: 12
    SSG: 15,3
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  • 5
    In: Journal of Ethnopharmacology, Elsevier BV, Vol. 262 ( 2020-11), p. 113214-
    Type of Medium: Online Resource
    ISSN: 0378-8741
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2020
    detail.hit.zdb_id: 1491279-X
    SSG: 15,3
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  • 6
    Online Resource
    Online Resource
    Mary Ann Liebert Inc ; 2021
    In:  The Journal of Alternative and Complementary Medicine Vol. 27, No. 1 ( 2021-01-01), p. 24-37
    In: The Journal of Alternative and Complementary Medicine, Mary Ann Liebert Inc, Vol. 27, No. 1 ( 2021-01-01), p. 24-37
    Type of Medium: Online Resource
    ISSN: 1075-5535 , 1557-7708
    Language: English
    Publisher: Mary Ann Liebert Inc
    Publication Date: 2021
    detail.hit.zdb_id: 2030749-4
    SSG: 5,21
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  • 7
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2013
    In:  Journal of Huazhong University of Science and Technology [Medical Sciences] Vol. 33, No. 1 ( 2013-2), p. 153-158
    In: Journal of Huazhong University of Science and Technology [Medical Sciences], Springer Science and Business Media LLC, Vol. 33, No. 1 ( 2013-2), p. 153-158
    Type of Medium: Online Resource
    ISSN: 1672-0733 , 1993-1352
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2013
    detail.hit.zdb_id: 2376511-2
    detail.hit.zdb_id: 2931065-9
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  • 8
    In: Molecular Therapy, Elsevier BV, Vol. 29, No. 7 ( 2021-07), p. 2268-2280
    Type of Medium: Online Resource
    ISSN: 1525-0016
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 2001818-6
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  • 9
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2022
    In:  Chinese Journal of Mechanical Engineering Vol. 35, No. 1 ( 2022-12)
    In: Chinese Journal of Mechanical Engineering, Springer Science and Business Media LLC, Vol. 35, No. 1 ( 2022-12)
    Abstract: With the rapid developments of marine resource exploitation, mounts of marine engineering equipment are settled on the ocean. When it is not possible to move the damaged equipment into a dry dock, welding operations must be performed in underwater environments. The underwater laser welding/cladding technique is a promising and advanced technique which could be widely applied to the maintenance of the damaged equipment. The present review paper aims to present a critical analysis and engineering overview of the underwater laser welding/cladding technique. First, we elaborated recent advances and key issues of drainage nozzles all over the world. Next, we presented the underwater laser processing and microstructural-mechanical behavior of repaired marine materials. Then, the newly developed powder-feeding based and wire-feeding based underwater laser direct metal deposition techniques were reviewed. The differences between the convection, conduction, and the metallurgical kinetics in the melt pools during underwater laser direct metal deposition and in-air laser direct metal deposition were illustrated. After that, several challenges that need to be overcame to achieve the full potential of the underwater laser welding/cladding technique are proposed. Finally, suggestions for future directions to aid the development of underwater laser welding/cladding technology and underwater metallurgical theory are provided. The present review will not only enrich the knowledge in the underwater repair technology, but also provide important guidance for the potential applications of the technology on the marine engineering.
    Type of Medium: Online Resource
    ISSN: 1000-9345 , 2192-8258
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2093153-0
    SSG: 6,25
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  • 10
    In: Orthopaedic Surgery, Wiley, Vol. 12, No. 5 ( 2020-10), p. 1495-1502
    Abstract: To evaluate the morphological similarity of bilateral coronoid process. Methods A total of 128 sets of computed tomography images of bilateral coronoid process from patients between January 2015 and December 2016 were acquired for three‐dimensional reconstruction to generate a coronoid process model. The patients were aged between 31.4 ± 9.3 years. The upper 40% of the coronoid process was trimmed as targeted fragment for morphological analysis. The height, length, width as well as the radius of the medial and lateral facet of the targeted fragment were compared in terms of laterality, age, and gender. To evaluate the similarity of the articular surface of the coronoid process, a local coordinate was created and coordinate transformation algorithm was developed to realign the bilateral coronoid process for the following matching. Then Delaunay triangulation was introduced for calculation of the area of the articular surface. After matching of articular surface of the upper 40% of bilateral coronoid process, the overlapping area of the articular surface was quantified to assess the similarity in morphology and compared in regard to age and gender. Results In this study, the height of the target fragment was 12.40 ± 2.74 mm, which was 12.62 ± 2.06 mm for male patients and 12.13 ± 3.76 mm for female patients ( t = 0.94, P = 0.35). The height of the target fragment was 12.79 ± 1.76 mm for patients 〉 40 years and 13.23 ± 3.16 mm for patients 〈 40 years ( t = 1.11, P = 0.27). The height of the target fragment of left and right coronoid process was 12.26 ± 3.40 mm and 12.74 ± 2.79 mm ( t = 1.15, P = 0.25). The length of the target fragment was 23.81 ± 2.67 mm, which was 23.86 ± 2.11 mm for male patients and 23.76 ± 2.85 mm for female patients ( t = 0.23, P = 0.82). The length of the target fragment was 22.92 ± 1.96 mm for patients 〉 40 years and 23.23 ± 2.14 mm for patients 〈 40 years ( t = 0.76, P = 0.45). The length of the target fragment of left and right coronoid process was 22.52 ± 2.89 mm and 21.66 ± 3.01 mm, respectively ( t = 1.00, P = 0.32). The width of the target fragment was 23.12 ± 1.92 mm on average, which was 23.06 ± 1.54 mm for male patients and 23.19 ± 2.82 mm for female patients ( t = 0.33, P = 0.74). The width of the target fragment was 24.82 ± 2.23 mm for patients 〉 40 years and 23.46 ± 3.38 mm for patients 〈 40 years ( t = 1.56, P = 0.12). The width of target fragment of left and right coronoid process was 24.42 ± 2.22 mm and 24.47 ± 2.69 mm, respectively ( t = 1.31, P = 0.19). The radius of medial facet was 6.44 ± 1.01 mm, which was 6.41 ± 1.39 mm for male patients and 6.47 ± 0.95 mm for female patients ( t = 0.28, P = 0.78). The radius of medial facet was 6.82 ± 1.28 mm for patients 〉 40 years and 6.46 ± 0.94 mm for patients 〈 40 years ( t = 1.31, P = 0.19). The radius of medial facet of left and right coronoid process was 6.43 ± 1.24 mm and 6.64 ± 1.34 mm ( t = 1.60, P = 0.11). The radius of lateral facet was 11.84 ± 3.71 mm, which was 11.61 ± 4.24 mm for male patients and 12.11 ± 3.09 mm for female patients ( t = 0.74, P = 0.46). The radius of medial facet was 11.82 ± 3.28 mm for patients 〉 40 years and 12.46 ± 3.94 mm for patients 〈 40 years ( t = 1.02, P = 0.31). The radius of lateral facet of left and right coronoid process was 11.97 ± 5.31 mm and 10.29 ± 3.29 mm, respectively ( t = 1.70, P = 0.09). The covering percentage of the articular surface of the upper 40% of bilateral coronoid process was 87% ± 12% with the covering percentage as 85.3% ± 14.2% for male patients and 90.0% ± 11.2% for female patients ( t = 0.75, P = 0.41). The covering percentage was 88.2% ± 11.7% for patients 〉 40 years and it was 87.4% ± 13.2% for patients 〈 40 years ( t = 0.98, P = 0.33). Conclusions The present study suggested that bilateral coronoid process shares high similarity in terms of 3D structure and articular surface morphology, which suggested that the osseous architecture of the coronoid process with comminuted fracture could be predicted by the morphological information of the contralateral side.
    Type of Medium: Online Resource
    ISSN: 1757-7853 , 1757-7861
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2483883-4
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