GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Lu, Lixin  (4)
Material
Publisher
Person/Organisation
Language
Years
  • 1
    Online Resource
    Online Resource
    Improved Genetic Map and Localization of Quantitative Trait Loci for Quality Traits in Auricularia heimuer
    MDPI AG ; 2023
    In:  Horticulturae Vol. 9, No. 7 ( 2023-07-02), p. 763-
    Details
    In: Horticulturae, MDPI AG, Vol. 9, No. 7 ( 2023-07-02), p. 763-
    Abstract: Auricularia heimuer is among China’s most important edible mushrooms and is rich in gum. With the improvement of people’s quality of life, demand is increasing for high-quality and good-tasting food; thus, the texture of A. heimuer is the focus of increasing attention. In this study, we added extra markers to a previously constructed genetic linkage map to generate a high-density genetic linkage map of A. heimuer, resolved the attributes of substrate quality-related traits, and performed quantitative trait locus (QTL) localization. The original genetic linkage map was improved by adding two new linkage groups, merging seven linkage groups into three linkage groups, and increasing the average linkage distance and total linkage estimated length. We anchored the 142 scaffolds of the genome to the improved genetic linkage map. In total, 15 significant QTLs controlling four quality-related traits were detected. Gumminess and chewiness, and cohesiveness and resilience, were linked. Three genes controlled cohesiveness and resilience; one gene controlled gumminess and chewiness. In conclusion, this study lays the foundation for gene localization and chromosome assembly in A. heimuer, elucidation of the mechanism of substrate quality-related trait formation, and provides a basis for precision breeding of A. heimuer.
    Type of Medium: Online Resource
    ISSN: 2311-7524
    URL: Article
    DOI: 10.3390/horticulturae9070763
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2813983-5
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Data_Sheet_1.docx
    Frontiers Media SA ; 2023
    In:  Frontiers in Microbiology Vol. 14 ( 2023-6-15)
    Details
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 14 ( 2023-6-15)
    Abstract: Owing to its great market potential for food and health care, white Auricularia cornea , a rare edible fungus, has received increased attention in recent years. This study presents a high-quality genome assembly of A. cornea and multi-omics analysis of its pigment synthesis pathway. Continuous Long Reads libraries, combined with Hi-C-assisted assembly were used to assemble of white A. cornea . Based on this data, we analyzed the transcriptome and metabolome of purple and white strains during the mycelium, primordium, and fruiting body stages. Finally, we obtained the genome of A.cornea assembled from 13 clusters. Comparative and evolutionary analysis suggests that A.cornea is more closely related to Auricularia subglabra than to Auricularia heimuer . The divergence of white/purple A.cornea occurred approximately 40,000 years ago, and there were numerous inversions and translocations between homologous regions of the two genomes. Purple strain synthesized pigment via the shikimate pathway. The pigment in the fruiting body of A. cornea was γ-glutaminyl-3,4-dihydroxy-benzoate. During pigment synthesis, α-D-glucose-1P, citrate, 2-Oxoglutarate, and glutamate were four important intermediate metabolites, whereas polyphenol oxidase and other 20 enzyme genes were the key enzymes. This study sheds light on the genetic blueprint and evolutionary history of the white A.cornea genome, revealing the mechanism of pigment synthesis in A.cornea . It has important theoretical and practical implications for understanding the evolution of basidiomycetes, molecular breeding of white A.cornea , and deciphering the genetic regulations of edible fungi. Additionally, it provides valuable insights for the study of phenotypic traits in other edible fungi.
    Type of Medium: Online Resource
    ISSN: 1664-302X
    URL: Article
    URL: Article
    DOI: 10.3389/fmicb.2023.1211795
    DOI: 10.3389/fmicb.2023.1211795.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2587354-4
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Dicarboxylic Amino Acid Permease 7219 Regulates Fruiting Body Type of Auricularia heimuer
    MDPI AG ; 2023
    In:  Journal of Fungi Vol. 9, No. 9 ( 2023-08-25), p. 876-
    Details
    In: Journal of Fungi, MDPI AG, Vol. 9, No. 9 ( 2023-08-25), p. 876-
    Abstract: Auricularia heimuer is a widely cultivated jelly mushroom. The fruiting bodies are categorized into cluster and chrysanthemum types. With changing consumer demands and the need to reduce bio-waste, the demand for clustered fruiting bodies is increasing. Therefore, gene mining for fruiting body types is a matter of urgency. We determined that the A. heimuer locus for fruiting body type was located at one end of the genetic linkage map. The locus was localized between the markers D23860 and D389 by increasing the density of the genetic linkage map. BlastN alignment showed that the marker SCL-18 was also located between D23860 and D389, and a total of 25 coding genes were annotated within this interval. Through parental transcriptome analysis and qRT-PCR verification, the locus g7219 was identified as the gene controlling the fruiting body type. A single-nucleotide substitution in the TATA box of g7219 was detected between the parents. By PCR amplification of the promoter region of g7219, the TATA-box sequences of the cluster- and chrysanthemum-type strains were found to be CATAAAA and TATAAAA, respectively. This study provides a foundation for the breeding of fruiting body types and strain improvement of A. heimuer.
    Type of Medium: Online Resource
    ISSN: 2309-608X
    URL: Article
    DOI: 10.3390/jof9090876
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2784229-0
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Velvet Family Members Regulate Pigment Synthesis of the Fruiting Bodies of Auricularia cornea
    MDPI AG ; 2023
    In:  Journal of Fungi Vol. 9, No. 4 ( 2023-03-27), p. 412-
    Details
    In: Journal of Fungi, MDPI AG, Vol. 9, No. 4 ( 2023-03-27), p. 412-
    Abstract: Color is a crucial feature to consider when breeding and improving strains of Auricularia cornea. To uncover the mechanism of white strain formation in A. cornea, this study selected parental strains that were homozygous for the color trait and analyzed the genetic laws of A. cornea color through genetic population construction, such as test-cross, back-cross, and self-cross populations, and the statistical analysis of color trait segregation. Moreover, the study developed SSR molecular markers to construct a genetic linkage map, perform the fine mapping the color-controlling genetic locus, and verify candidate genes using yeast two-hybrid, transcriptome analysis, and different light treatments. The results of the study indicated that the color trait of A. cornea is controlled by two pairs of alleles. When both pairs of loci are dominant, the fruiting body is purple, while when both pairs of loci are recessive or one pair of loci is recessive, the fruiting body is white. Based on the linkage map, the study finely mapped the color locus within Contig9_29,619bp-53,463bp in the A. cornea genome and successfully predicted the color-controlling locus gene A18078 (AcveA), which belongs to the Velvet factor family protein and has a conserved structure domain of the VeA protein. It can form a dimer with the VelB protein to inhibit pigment synthesis in filamentous fungi. Lastly, the study validated the interaction between AcVeA and VelB (AcVelB) in A. cornea at the gene, protein, and phenotype levels, revealing the mechanism of inhibition of pigment synthesis in A. cornea. Under dark conditions, dimerization occurs, allowing it to enter the nucleus and inhibit pigment synthesis, leading to a lighter fruiting body color. However, under light conditions, the dimer content is low and cannot enter the nucleus to inhibit pigment synthesis. In summary, this study clarified the mechanism of white strain formation in A. cornea, which could aid in improving white strains of A. cornea and studying the genetic basis of color in other fungi.
    Type of Medium: Online Resource
    ISSN: 2309-608X
    URL: Article
    DOI: 10.3390/jof9040412
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2784229-0
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...