In:
Frontiers in Molecular Neuroscience, Frontiers Media SA, Vol. 14 ( 2021-9-23)
Abstract:
Recurrent chest blast exposure can lead to brain inflammation, oxidative stress, and mental disorders in soldiers. However, the mechanism that underlies brain injury caused indirectly by chest blasts remains unclear. It is urgent to find additional reliable biomarkers to reveal the intimate details of the pathogenesis of this phenomenon. We used the term tandem mass tag (TMT) labeling combined with liquid chromatography–tandem mass spectrometry (LC-MS/MS) to screen for differentially expressed proteins in rat brain at different time points after a chest blast. Data are available via ProteomeXchange with the identifier PXD025204. Gene Ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG), the Database for Annotation, Visualization and Integrated Discovery (DAVID), and Cytoscape analyses were used to analyze the proteomic profiles of blast-exposed rats. In addition, we performed Western blotting to verify protein levels. We identified 6,931 proteins, of which 255 were differentially expressed and 43, 84, 52, 97, and 49 were identified in brain tissues at 12, 24, 48, and 72 h and 1 week after chest blast exposure, respectively. In this study, the GO, KEGG, Clusters of Orthologous Groups of proteins, and Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) analyses indicated that brain damage caused by chest blast exposure involved many important biological processes and signaling pathways, such as inflammation, cell adhesion, phagocytosis, neuronal and synaptic damage, oxidative stress, and apoptosis. Furthermore, Western blotting confirmed that these differentially expressed proteins and affected signaling pathways were associated with brain damage caused by chest blast exposure. This study identifies potential protein biomarkers of brain damage caused indirectly by chest blast and new targets for the treatment of this condition.
Type of Medium:
Online Resource
ISSN:
1662-5099
DOI:
10.3389/fnmol.2021.688050
DOI:
10.3389/fnmol.2021.688050.s001
DOI:
10.3389/fnmol.2021.688050.s002
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10.3389/fnmol.2021.688050.s003
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10.3389/fnmol.2021.688050.s004
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10.3389/fnmol.2021.688050.s005
DOI:
10.3389/fnmol.2021.688050.s006
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10.3389/fnmol.2021.688050.s007
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10.3389/fnmol.2021.688050.s008
DOI:
10.3389/fnmol.2021.688050.s009
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10.3389/fnmol.2021.688050.s010
DOI:
10.3389/fnmol.2021.688050.s011
DOI:
10.3389/fnmol.2021.688050.s012
DOI:
10.3389/fnmol.2021.688050.s013
DOI:
10.3389/fnmol.2021.688050.s014
DOI:
10.3389/fnmol.2021.688050.s015
DOI:
10.3389/fnmol.2021.688050.s016
DOI:
10.3389/fnmol.2021.688050.s017
DOI:
10.3389/fnmol.2021.688050.s018
DOI:
10.3389/fnmol.2021.688050.s019
DOI:
10.3389/fnmol.2021.688050.s020
DOI:
10.3389/fnmol.2021.688050.s021
DOI:
10.3389/fnmol.2021.688050.s022
DOI:
10.3389/fnmol.2021.688050.s023
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2452967-9
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