In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 7 ( 2022-7-28), p. e0269102-
Abstract:
Avian pathogenic Escherichia coli (APEC), which has potential zoonotic risk, can cause severe systemic infections such as septicemia and meningitis in poultry. Colibactin is a hybrid non-ribosomal peptide/polyketide secondary metabolite produced by bacteria, which induces double-strand DNA breaks and chromosome instability in eukaryotic cells. ClbA is a 4’-phosphopantetheinyl transferase (PPTase) that is essential for colibactin and plays a role in siderophore synthesis. However, whether ClbA is associated with meningitis development in APEC is unclear. In this study, we abolished the clbA gene in the APEC XM strain, investigated the effect of clbA on colibactin synthesis and evaluated the pathogenic capacity of colibactin on meningitis development. Deletion of clbA reduced DNA damage to cells and hindered the normal synthesis of colibactin. Compared with the mice infected by wild-type APEC XM, the clbA deletion mutant infected mice had significant reduction in a series of characteristics associated with meningitis including clinical symptoms, bacterial loads of blood and brain, disruption of the blood brain barrier and the expression of inflammatory factors in the brain tissue. Complementation of ClbA recovered some APEC XM virulence. We conclude that ClbA is obligatory for the synthesis of colibactin and is responsible for the development of meningitis in mice infected by APEC.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0269102
DOI:
10.1371/journal.pone.0269102.g001
DOI:
10.1371/journal.pone.0269102.g002
DOI:
10.1371/journal.pone.0269102.g003
DOI:
10.1371/journal.pone.0269102.g004
DOI:
10.1371/journal.pone.0269102.g005
DOI:
10.1371/journal.pone.0269102.g006
DOI:
10.1371/journal.pone.0269102.g007
DOI:
10.1371/journal.pone.0269102.g008
DOI:
10.1371/journal.pone.0269102.g009
DOI:
10.1371/journal.pone.0269102.t001
DOI:
10.1371/journal.pone.0269102.s001
DOI:
10.1371/journal.pone.0269102.s002
DOI:
10.1371/journal.pone.0269102.s003
DOI:
10.1371/journal.pone.0269102.s004
DOI:
10.1371/journal.pone.0269102.s005
DOI:
10.1371/journal.pone.0269102.s006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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