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  • Ovid Technologies (Wolters Kluwer Health)  (2)
  • Liu, Xuanyou  (2)
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  • Ovid Technologies (Wolters Kluwer Health)  (2)
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  • 1
    Online Resource
    Online Resource
    Abstract 621: Basic Fibroblast Growth Factor Helps to Maintain the Stemness of Rat Multipotent Adult Progenitor Cells Partially via Erk1/2 Signaling
    Ovid Technologies (Wolters Kluwer Health) ; 2017
    In:  Arteriosclerosis, Thrombosis, and Vascular Biology Vol. 37, No. suppl_1 ( 2017-05)
    Details
    In: Arteriosclerosis, Thrombosis, and Vascular Biology, Ovid Technologies (Wolters Kluwer Health), Vol. 37, No. suppl_1 ( 2017-05)
    Abstract: Bone marrow mesenchymal stem cells are one of the important sources for cell replacement therapies. The outcome of the cell-based therapies is determined by a variety of factors including the source of the cells and their functional status. The functional status is usually measured by the expression of stem cell specific marker Oct-4 (stemness), differentiation potential (multipotency), and production of paracrine factors. It could be beneficial to keep the stem cells in a multipotent state for their optimal therapeutic outcome. The present study was to investigate the effect of basic fibroblast growth factor (bFGF) on the stemness of bone marrow stem cells and related mechanism. Rat multipotent adult progenitor cells (MAPCs) were used as the source of bone marrow stem cells, and induced to differentiate in vitro with (bFGF:5ng/ml and 20ng/ml) and without bFGF for up to 7 days. The expression of Oct4 and endothelial markers including Flk1, VWF and CD31, and smooth muscle cell markers including α-SMA, SM22 and CNN1 were determined with real-time (RT) PCR and western blot. We observed that both transcriptional (as reflected by RT-PCR) and protein expression (by western blot) of Oct4 was maintained at relatively stable levels in the cells treated with bFGF during the early phase of differentition. On the other hand, the expression of Flk1, VWF, CD31 and α-SMA, SM22, CNN1 were significantly decreased in the cells with the presence of bFGF. Treatment with bFGF significantly increased the phosphorylation of Erk1/2, not Akt nor STAT3, in the cells. Inhibition of Erk1/2 phosphorylation with PD98059 partially but significantly attenuated the effect of bFGF on Oct4 expression in the cells. These data suggest that bFGF could be able to maintain the bone marrow stem cells in their undifferentiated state in vitro partially through Erk1/2 signaling-mediated mechanism.
    Type of Medium: Online Resource
    ISSN: 1079-5642 , 1524-4636
    URL: Article
    DOI: 10.1161/atvb.37.suppl_1.621
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2017
    detail.hit.zdb_id: 1494427-3
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  • 2
    Online Resource
    Online Resource
    Abstract 201: High Fat Diet Selectively Decreases the Bone Marrow Lin-/c-kit+ Cell Population in Aging Mice Through Increased ROS Production
    Ovid Technologies (Wolters Kluwer Health) ; 2017
    In:  Circulation Research Vol. 121, No. suppl_1 ( 2017-07-21)
    Details
    In: Circulation Research, Ovid Technologies (Wolters Kluwer Health), Vol. 121, No. suppl_1 ( 2017-07-21)
    Abstract: Bone marrow-derived c-kit-positive (c-kit + ) cells are a potential source for cell-based therapy and tissue repair and regeneration. The ability of tissue repair and regeneration is significantly decreased in the aging population for various reasons. Western diet usually has increased level of fat. The present study was designed to evaluate the effect of long-term high fat diet on bone marrow c-Kit positive cells and the role of ROS in aging mice. Aging male wild-type (WT) C57BL/6 mice (40 weeks) were fed with high fat diet (HFD) for 3 months with regular diet as the control. To evaluate the role of reactive oxygen species (ROS) in mediating the effect of HFD on bone marrow cell population, a separate group of mice were treated with N-acetylcysteine (NAC) to reduce ROS production. Bone marrow (BM) and blood cells were harvested and prepared in the mice after 3 months of HFD treatment for flowcytometry analysis for Sca-1+, or c-Kit+, or CD133+ cells. The lineage negative (Lin-) and c-Kit positive (Lin-/c-Kit+) cell population was significantly decreased in the BM, not in blood, in the mice with HFD, while no significant change was observed in Sca-1+ or CD133+ cell populations in the BM or blood. The BM Lin-/c-Kit+ cells also exhibited increased intracellular ROS level, increased level of apoptosis, and deceased level of proliferation in HFD-treated mice. NAC treatment significantly decreased intracellular ROS level and normalized the BM Lin-/c-Kit+ cell population in HFD mice. In conclusion, the present study demonstrated that long-term HFD selectively decreased BM Lin-/c-Kit+ cell population in the aging mice through increased ROS production.
    Type of Medium: Online Resource
    ISSN: 0009-7330 , 1524-4571
    URL: Article
    DOI: 10.1161/res.121.suppl_1.201
    RVK:
    XA 10000
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2017
    detail.hit.zdb_id: 1467838-X
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