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  • 1
    Online Resource
    Online Resource
    P53-induced GAP-43 Upregulation in Primary Cortical Neurons of Rats
    Bentham Science Publishers Ltd. ; 2024
    In:  Protein & Peptide Letters Vol. 31, No. 3 ( 2024-03), p. 229-235
    Details
    In: Protein & Peptide Letters, Bentham Science Publishers Ltd., Vol. 31, No. 3 ( 2024-03), p. 229-235
    Abstract: In this study, we employed an in vitro culturing technique to investigate the impact of p53 on the modulation of growth-associated protein-43 (GAP-43) within the primary cortical neurons of rat specimens. Methods: (1) Within the first 24 hours after birth, the bilateral cortex was extracted from newborn Wistar rats and primary cortical neurons were cultured and identified. (2) The changes in the mRNA and protein expressions of GAP-43 induced by p53 in rat primary cortical neurons cultured in vitro were identified utilizing real-time polymerase chain reaction and western blot techniques. Results: (1) Lentiviral transfection of p53 within primary cortical neurons of rats elicited elevated levels of both mRNA and protein expressions of GAP-43, consequently culminating in a noteworthy augmentation of p53 expression. (2) The introduction of a p53 inhibitor in rat primary cortical neurons resulted in a reduction in both mRNA and protein expressions of GAP-43. Conclusion: Within primary rat cortical neurons, p53 has the potential to prompt an augmentation in both the transcriptional and protein expression levels of the GAP-43 protein.
    Type of Medium: Online Resource
    ISSN: 0929-8665
    URL: Article
    DOI: 10.2174/0109298665263864231221071712
    Language: English
    Publisher: Bentham Science Publishers Ltd.
    Publication Date: 2024
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  • 2
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    Repurposing Erythrocytes as a “Photoactivatable Bomb”: A General Strategy for Site‐Specific Drug Release in Blood Vessels
    Wiley ; 2021
    In:  Small Vol. 17, No. 34 ( 2021-08)
    Details
    In: Small, Wiley, Vol. 17, No. 34 ( 2021-08)
    Abstract: Tumor vasculature has long been considered as an extremely valuable therapeutic target for cancer therapy, but how to realize controlled and site‐specific drug release in tumor blood vessels remains a huge challenge. Despite the widespread use of nanomaterials in constructing drug delivery systems, they are suboptimal in principle for meeting this demand due to their easy blood cell adsorption/internalization and short lifetime in the systemic circulation. Here, natural red blood cells (RBCs) are repurposed as a remote‐controllable drug vehicle, which retains RBC's morphology and vessel‐specific biodistribution pattern, by installing photoactivatable molecular triggers on the RBC membrane via covalent conjugation with a finely tuned modification density. The molecular triggers can burst the RBC vehicle under short and mild laser irradiation, leading to a complete and site‐specific release of its payloads. This cell‐based vehicle is generalized by loading different therapeutic agents including macromolecular thrombin, a blood clotting‐inducing enzyme, and a small‐molecule hypoxia‐activatable chemodrug, tirapazamine. In vivo results demonstrate that the repurposed “anticancer RBCs” exhibit long‐term stability in systemic circulation but, when tumors receive laser irradiation, precisely releases their cargoes in tumor vessels for thrombosis‐induced starvation therapy and local deoxygenation‐enhanced chemotherapy. This study proposes a general strategy for blood vessel‐specific drug delivery.
    Type of Medium: Online Resource
    ISSN: 1613-6810 , 1613-6829
    URL: Issue
    URL: Article
    DOI: 10.1002/smll.v17.34
    DOI: 10.1002/smll.202100753
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2168935-0
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  • 3
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    Cationic Liposomes with Different Lipid Ratios: Antibacterial Activity, Antibacterial Mechanism, and Cytotoxicity Evaluations
    MDPI AG ; 2022
    In:  Pharmaceuticals Vol. 15, No. 12 ( 2022-12-14), p. 1556-
    Details
    In: Pharmaceuticals, MDPI AG, Vol. 15, No. 12 ( 2022-12-14), p. 1556-
    Abstract: Due to their strong bacterial binding and bacterial toxicity, cationic liposomes have been utilized as effective antibacterial materials in many studies. However, few researchers have systematically compared their antibacterial activity with their mammalian cell cytotoxicity or have deeply explored their antibacterial and cytotoxicity mechanisms. Here, we prepared a series of cationic liposomes (termed CLs) using dimethyldioctadecylammonium chloride (DODAC) and lecithin at different molar ratios. CLs have the ability to effectively bind with Gram-positive and Gram-negative bacteria through electrostatic and hydrophobic interactions. Further, the CLs with high molar ratios of DODAC (30 and 40 mol%) can disrupt the bacterial wall/membrane, efficiently inducing the production of reactive oxygen species (ROS). More importantly, we carefully compared the antibacterial activity and the mammalian cell cytotoxicity of various CLs differing in DODAC contents and liposomal concentrations and revealed that, whether they are bacterial or mammalian cells, an increasing DODAC content in CLs can lead to an elevated cytotoxicity level. Further, there exists a critical DODAC contents ( 〉 20 mol%) in CLs to endow them with effective antibacterial ability. However, the variation in the DODAC content and liposomal concentration of CLs has different degrees of influence on the antibacterial activity or cytotoxicity. For example, CLs at high DODAC content (i.e., CL0.3 and CL0.4) could effectively kill both types of bacterial cells but only cause negligible toxicity to mammalian cells. We believe that a systematic comparison between the antibacterial activity and the cytotoxicity of CLs with different DODAC contents will provide an important reference for the potential clinical applications of cationic liposomes.
    Type of Medium: Online Resource
    ISSN: 1424-8247
    URL: Article
    DOI: 10.3390/ph15121556
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2193542-7
    SSG: 15,3
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  • 4
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    Sparse Bayesian predictive modelling of tumour response using radiomic features
    Wiley ; 2022
    In:  Stat Vol. 11, No. 1 ( 2022-12)
    Details
    In: Stat, Wiley, Vol. 11, No. 1 ( 2022-12)
    Abstract: We propose a sparse Bayesian hierarchical model for the analysis of data including radiomic features for characterization of head and neck squamous cell carcinoma. The proposed model facilitates radiomic feature selection, handling of missing values in key predictors as well as prediction in a unified framework. The fully Bayesian approach enables adequate incorporation of uncertainty arising from various aspects of the inference and prediction procedure. The prediction performance of the model is assessed via cross validation and compared with two frequentist methods.
    Type of Medium: Online Resource
    ISSN: 2049-1573 , 2049-1573
    URL: Issue
    URL: Article
    DOI: 10.1002/sta4.v11.1
    DOI: 10.1002/sta4.450
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2687133-6
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  • 5
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    Enzymatic Nanosphere‐to‐Nanofiber Transition and Autophagy Inducer Release Promote Tumor Chemotherapy
    Wiley ; 2022
    In:  Advanced Healthcare Materials Vol. 11, No. 23 ( 2022-12)
    Details
    In: Advanced Healthcare Materials, Wiley, Vol. 11, No. 23 ( 2022-12)
    Abstract: Chemotherapy has remained an effective and predominant cancer treatment for the past decades, but is hampered by its low response rate and severe systemic toxicity. Combination chemotherapies are proposed to address these issues, yet their therapeutic outcomes are still far from satisfactory. Thus, it is urgent to develop novel strategies to promote tumor chemosensitivity while reducing toxic side effects of chemotherapeutics. Herein, employing a rationally designed peptide conjugate Nap‐Phe‐Phe‐Lys(SA‐AZD8055)‐Tyr(H 2 PO 3 )‐OH ( Nap‐AZD‐Yp ), a novel approach of simultaneous intracellular nanofiber formation and autophagy inducer release is proposed for selectively sensitizing tumor to chemotherapy. Upon sequential catalyses of alkaline phosphatase and carboxylesterase, Nap‐AZD‐Yp undergoes nanosphere‐to‐nanofiber transition accompanied by autophagy inducer AZD8055 release in cancer cells. Cell experiments show enhanced endocytosis of anticancer drug doxorubicin and inhibition of cell migration due to the intracellular nanofiber formation. The released AZD8055 further activates excessive autophagy of cancer cells, sensitizing them to chemotherapy. Animal experiment results suggest Nap‐AZD‐Yp can significantly enhance the therapeutic effects of doxorubicin on tumors while mitigate its toxic adverse effects on normal tissues. It is anticipated that the “smart” concept in this work c be widely employed to develop novel combinational therapies for the treatment of cancers and other diseases in near future.
    Type of Medium: Online Resource
    ISSN: 2192-2640 , 2192-2659
    URL: Issue
    URL: Article
    DOI: 10.1002/adhm.v11.23
    DOI: 10.1002/adhm.202201916
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2645585-7
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  • 6
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    Identification of heterotic loci associated with grain yield and its components using two CSSL test populations in maize
    Springer Science and Business Media LLC ; 2016
    In:  Scientific Reports Vol. 6, No. 1 ( 2016-12-05)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 6, No. 1 ( 2016-12-05)
    Abstract: Heterosis has widely been used to increase grain yield and quality. In this study, the genetic basis of heterosis on grain yield and its main components in maize were examined over 2 years in two locations in two test populations constructed from a set of 184 chromosome segment substitution lines (CSSLs) and two inbred lines (Zheng58 and Xun9058). Of the 169 heterotic loci (HL) associated with grain yield and its five components identified in CSSL × Zheng58 and CSSL × Xun9058 test populations, only 25 HL were detected in both populations. The comparison of quantitative trait loci (QTLs) detected in the CSSL population with HL detected in the two test populations revealed that only 15.46% and 17.35% of the HL in the given populations respectively, shared the same chromosomal regions as that of the corresponding QTLs and showed dominant effects as well as pleiotropism with additive and dominant effects. In addition, most of the HL (74.23% and 74.49%) had overdominant effects. These results suggest that overdominance is the main contributor to the effects of heterosis on grain yield and its components in maize, and different HL are associated with heterosis for different traits in different hybrids.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/srep38205
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2016
    detail.hit.zdb_id: 2615211-3
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  • 7
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    Development and validation of the diabetic self-management scale based on information-motivation-behavioral skills theory
    Frontiers Media SA ; 2023
    In:  Frontiers in Public Health Vol. 11 ( 2023-2-24)
    Details
    In: Frontiers in Public Health, Frontiers Media SA, Vol. 11 ( 2023-2-24)
    Abstract: Self-management is important for the blood sugar control of middle-aged and elderly Type 2 diabetes mellitus (T2DM) patients, of which diet, exercise, and drug compliance are the most common components. The Information-Motivation-Behavioral Skills Model (IMB) has been widely used in health behavior management and intervention. Objective The purpose of this study is to develop and validate the Diabetic Self-Management Scale (DSMS) based on the IMB model. Methods Self-report survey data was collected from middle-aged and elderly T2DM patients in Zhongmu City, Henan Province, China in November 2021 using convenience sampling. The original DSMS was developed through a literature review and summary of previous similar scales using an inductive approach. Item modification was finished by a panel of specialists. Exploratory factor analysis and confirmatory factor analysis were used to evaluate the reliability, convergent validity, discriminant validity, and criterion validity of DSMS. Results Four hundred and sixty nine T2DM patients completed the questionnaire survey. The final DSMS consists of 22 items with three dimensions, including information (five items), motivation (eight items), and behavior skills (nine items). The results of simple factor analysis showed that the KMO value was 0.839, Bartlett spherical test 2 = 3254.872, P & lt; 0.001. The results of confirmatory factor analysis showed that 2/df = 2.261, RMSEA = 0.073, CFI = 0.937, TLI = 0.930, and SRMR = 0.096. The standardized factor loadings of 22 DSMS items were all above 0.6, and the CR values of 3 dimensions were all higher than 0.9. In addition, DSMS also showed good discriminant and criterion validity. Conclusion The 22-item DSMS has good reliability and validity, and can be used to make diabetic self-management assessment regarding diet, physical activity, and medication among middle-aged and elderly Chinese T2DM patients. DSMS is of moderate length and easy to understand. It can be promoted in China in the future to understand the self-management status of middle-aged and elderly T2DM patients in China.
    Type of Medium: Online Resource
    ISSN: 2296-2565
    URL: Article
    DOI: 10.3389/fpubh.2023.1109158
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2711781-9
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  • 8
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    Tandem Guest‐Host‐Receptor Recognitions Precisely Guide Ciprofloxacin to Eliminate Intracellular Staphylococcus aureus
    Wiley ; 2023
    In:  Angewandte Chemie International Edition Vol. 62, No. 32 ( 2023-08-07)
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 62, No. 32 ( 2023-08-07)
    Abstract: Staphylococcus aureus ( S. aureus ) is able to hide within host cells to escape immune clearance and antibiotic action, causing life‐threatening infections. To boost the therapeutic efficacy of antibiotics, new intracellular delivery approaches are urgently needed. Herein, by rational design of an adamantane (Ada)‐containing antibiotic‐peptide precursor Ada‐Gly‐Tyr‐Val‐Ala‐Asp‐Cys(StBu)‐Lys(Ciprofloxacin)‐CBT ( Cip‐CBT‐Ada ), we propose a strategy of tandem guest‐host‐receptor recognitions to precisely guide ciprofloxacin to eliminate intracellular S. aureus . Via guest‐host recognition, Cip‐CBT‐Ada is decorated with a β‐cyclodextrin‐heptamannoside ( CD‐M ) derivative to yield Cip‐CBT‐Ada/CD‐M , which is able to target mannose receptor‐overexpressing macrophages via multivalent ligand‐receptor recognition. After uptake, Cip‐CBT‐Ada/CD‐M undergoes caspase‐1 (an overexpressed enzyme during S. aureus infection)‐initiated CBT‐Cys click reaction to self‐assemble into ciprofloxacin nanoparticle Nano‐Cip . In vitro and in vivo experiments demonstrate that, compared with ciprofloxacin or Cip‐CBT‐Ada , Cip‐CBT‐Ada/CD‐M shows superior intracellular bacteria elimination and inflammation alleviation efficiency in S. aureus ‐infected RAW264.7 cells and mouse infection models, respectively. This work provides a supramolecular platform of tandem guest‐host‐receptor recognitions to precisely guide antibiotics to eliminate intracellular S. aureus infection efficiently.
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v62.32
    DOI: 10.1002/anie.202306427
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
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  • 9
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    Strategies for visualizing inflammation
    Wiley ; 2021
    In:  VIEW Vol. 2, No. 1 ( 2021-02)
    Details
    In: VIEW, Wiley, Vol. 2, No. 1 ( 2021-02)
    Abstract: As a basic innate immune response to the disordered tissue homeostasis, inflammation is related to the pathogenesis of multiple diseases, including bacterial infections, atherosclerosis, neurodegenerative diseases, and cancers. It is also a pivotal feature of some metabolic disorders such as diabetes and obesity. The visualization of in vivo inflammations can help us to comprehend the pathogenesis of these diseases and develop new solutions to diagnose them. Over the past few decades, a variety of strategies (eg, computed tomography, magnetic resonance imaging [MRI], and ultrasound [US] imaging) have been utilized for visualizing inflammations by imaging the structural changes of inflammatory tissues. Moreover, many recent studies have focused on some probes that can target or localize the inflammatory sites by specific binding to inflammation‐related molecules, being internalized by inflammatory cells, or becoming detectable only under inflammatory conditions. These probes can also be applied to visualize inflammations by MRI, positron emission tomography, single‐photon emission computed tomography, photoacoustic imaging, optical imaging (eg, fluorescence imaging, bioluminescence imaging, and chemiluminescence imaging), contrast‐enhanced US imaging, and the combined use of the abovementioned methods. This review not only summarizes the existing strategies for visualizing inflammations, but also discusses the limitations of the present strategies and the future directions on the development of new strategies for realizing the in vivo inflammation visualization.
    Type of Medium: Online Resource
    ISSN: 2688-268X , 2688-268X
    URL: Issue
    URL: Article
    DOI: 10.1002/viw2.v2.1
    DOI: 10.1002/VIW.20200025
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 3021474-9
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  • 10
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    Fluorescent dendrimer-based probes for cell membrane imaging: Zebrafish epidermal labeling-based toxicity evaluation
    Elsevier BV ; 2022
    In:  Biosensors and Bioelectronics Vol. 213 ( 2022-10), p. 114403-
    Details
    In: Biosensors and Bioelectronics, Elsevier BV, Vol. 213 ( 2022-10), p. 114403-
    Type of Medium: Online Resource
    ISSN: 0956-5663
    URL: Article
    DOI: 10.1016/j.bios.2022.114403
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2022
    detail.hit.zdb_id: 1496379-6
    SSG: 12
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