In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 63, No. Pt_1 ( 2013-01-01), p. 129-133
Abstract:
Two Gram-positive, rod-shaped, non-spore-forming bacteria (strains RHLT 1 -17 T and RHLT 2 -1 T ) were isolated from Hailuogou glacier in Szechwan province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the genus Nocardioides and shared 97.8 % sequence similarity with each other and 97.6 and 98.4 % sequence similarity, respectively, with Nocardioides kribbensis KSL-2 T . Strain RHLT 1 -17 T grew at 0–35 °C and strain RHLT 2 -1 T grew at 0–25 °C. The major cellular fatty acids of strain RHLT 1 -17 T were C 17 : 1 ω8 c (32.69 %) and iso-C 16 : 0 (21.74 %). The major cellular fatty acids of strain RHLT 2 -1 T were C 18 : 1 ω9 c (28.72 %), summed feature 3 (17.14 %; comprising C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ), iso-C 16 : 0 (14.35 %), C 16 : 0 (9.96 %) and iso-C 14 : 0 (8.34 %). Both strains contained ll -2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-8(H 4 ) as the predominant menaquinone. On the basis of data obtained using a polyphasic approach, two novel species, Nocardioides szechwanensis sp. nov. (type strain RHLT 1 -17 T = CGMCC 1.11147 T = NBRC 108562 T ) and Nocardioides psychrotolerans sp. nov. (type strain RHLT 2 -1 T = CGMCC 1.11156 T = NBRC 108563 T ), are proposed.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.038091-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2013
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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