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  • 1
    Online Resource
    Online Resource
    Study on bioactive molecules involved in extracellular biosynthesis of silver nanoparticles by Penicillium aculeatum Su1
    American Scientific Publishers ; 2019
    In:  Materials Express Vol. 9, No. 5 ( 2019-08-01), p. 475-483
    Details
    In: Materials Express, American Scientific Publishers, Vol. 9, No. 5 ( 2019-08-01), p. 475-483
    Abstract: In this paper, the biosynthesis of high-stable and biocompatible silver nanoparticles (AgNPs) was implemented by employing cell-free filtrate of Penicillium aculeatum Su1. The compositions analysis of reducing biomolecules in reaction system before and after AgNPs synthesis suggested that proteins were mainly involved in the biosynthesis process of AgNPs. Polyacrylamide gel electrophoresis (SDS-PAGE) analysis displayed that two main protein bands with molecular weights ranging from 66.2 to 116 KDa and 35 to 45 KDa were capped on the surface of AgNPs. The further identification of these protein bands by liquid chromatography-mass spectrometry (LC-MS/MS) analysis indicated that actin as a major protein component was responsible for stabilization of prepared AgNPs. The activity of nitrate reductase secreted by P. aculeatum Su1 was 73.73 ± 3.89 μ g/(g · h). Furthermore, the dialysis assay showed that small molecular components had significant impacts on yield and particle size of biosynthesized AgNPs. Reduced nicotinamide adenine dinucleotide or reduced nicotinamide adenine dinucleotide phosphate (NADH or NADPH)-dependent nitrate reductases and other types of reductases or non-enzymatic bioactive molecules (≥ 3.5 KDa) might simultaneously participate in the biosynthesis process of AgNPs mediated by P. aculeatum Su1.
    Type of Medium: Online Resource
    ISSN: 2158-5849
    URL: Article
    DOI: 10.1166/mex.2019.1508
    Language: English
    Publisher: American Scientific Publishers
    Publication Date: 2019
    detail.hit.zdb_id: 2905325-0
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  • 2
    Online Resource
    Online Resource
    Synergistic Antibacterial Effect from Silver Nanoparticles and Anticancer Activity Against Human Lung Cancer Cells
    American Scientific Publishers ; 2022
    In:  Journal of Biomedical Nanotechnology Vol. 18, No. 9 ( 2022-09-01), p. 2204-2215
    Details
    In: Journal of Biomedical Nanotechnology, American Scientific Publishers, Vol. 18, No. 9 ( 2022-09-01), p. 2204-2215
    Abstract: Microbially synthesized silver nanoparticles (AgNPs) with high stability and bioactivity have recently shown considerable promise in biomedical research and application. In this study, AgNPs prepared by Penicillium aculeatum Su1 exhibited effective antibacterial action by inhibiting bacterial growth and destroying cellular structure. Meanwhile, their assessed increased in fold area (IFA) through the Kirby-Bauer disc diffusion method proved that, the AgNPs showed synergistic antibacterial effect on different bacteria when combined with antibiotics, especially for drug-resistant P. aeruginosa (4.58∼6.36-fold) and B. subtilis (4.2-fold). Moreover, the CCK-8 assay and flow cytometric analysis were used to evaluate the cytotoxic effects of AgNPs on normal cells (HBE) and lung cancer cells (HTB-182), which confirmed that they presented higher biocompatibility towards HBE cells when compared with silver ions, but high cytotoxicity in a dosedependent manner with an IC 50 values of 35.00 μ g/mL towards HTB-182 cells by raising intracellular reactive oxygen species (ROS) levels, hindering cell proliferation, and ultimately leading to cell cycle arrest and cell apoptosis. These results demonstrate that, the biosynthesized AgNPs could be a potential candidate for future therapies of infection caused by drug-resistant bacteria, as well as lung squamous cell carcinoma.
    Type of Medium: Online Resource
    ISSN: 1550-7033
    URL: Article
    DOI: 10.1166/jbn.2022.3420
    Language: English
    Publisher: American Scientific Publishers
    Publication Date: 2022
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  • 3
    Online Resource
    Online Resource
    Preparation and Synergistic Antibacterial Effects of Poly(ethyleneimine) Stabilized Silver Antibacterial Agents for Light Colored Fabrics
    American Scientific Publishers ; 2016
    In:  Nanoscience and Nanotechnology Letters Vol. 8, No. 6 ( 2016-06-01), p. 471-477
    Details
    In: Nanoscience and Nanotechnology Letters, American Scientific Publishers, Vol. 8, No. 6 ( 2016-06-01), p. 471-477
    Type of Medium: Online Resource
    ISSN: 1941-4900 , 1941-4919
    URL: Article
    DOI: 10.1166/nnl.2016.2161
    Language: English
    Publisher: American Scientific Publishers
    Publication Date: 2016
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  • 4
    Online Resource
    Online Resource
    Extracellular Biosynthesis, Characterization and Cytotoxic Effect of Silver Nanoparticles by Streptomyces coelicoflavus KS-3
    American Scientific Publishers ; 2018
    In:  Journal of Nanoscience and Nanotechnology Vol. 18, No. 12 ( 2018-12-01), p. 8133-8141
    Details
    In: Journal of Nanoscience and Nanotechnology, American Scientific Publishers, Vol. 18, No. 12 ( 2018-12-01), p. 8133-8141
    Abstract: The present work aimed to investigate extracellular biosynthesis of silver nanoparticles (AgNPs) mediated by an actinomycete strain and their cytotoxic effects compared with silver ions. The selected strain was identified as Streptomyces coelicoflavus KS-3 by phenotypical characteristics and 16S rRNA analysis. The formation of biosynthesized AgNPs was proved by an absorption peak observed at 437 nm. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) analyses revealed that the prepared AgNPs were spherical or approximately spherical followed by a small amount of truncated triangular, quadrangular and hexagonal with the particle diameters ranging from 2.33 to 91.3 nm. X-ray diffraction (XRD) pattern confirmed that the AgNPs presented a face-centered cubic (FCC) structure of crystalline silver. Energy dispersive of X-ray (EDX) spectrum and Fourier transform infrared spectroscopy (FTIR) analyses verified the existence of biomolecules, such as proteins, that participated in the formation and stabilization of AgNPs. Furthermore, the comparative study on cytotoxic effect of AgNPs indicated that the AgNPs exhibited higher biocompatibility towards human bronchial epithelial (HBE) cells than silver ions and exerted potent cytotoxic effect in a dose-dependent manner against human lung squamous cell carcinoma cells (HTB-182) and human lung adenocarcinoma cells (A549) with the concentrations ranging from 1 to 50 μ g/mL.
    Type of Medium: Online Resource
    ISSN: 1533-4880
    URL: Article
    DOI: 10.1166/jnn.2018.16388
    Language: English
    Publisher: American Scientific Publishers
    Publication Date: 2018
    SSG: 11
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  • 5
    Online Resource
    Online Resource
    Fast iterative solver for 3D finite-element modeling of controlled-source electromagnetic responses with vector and scalar potentials
    Society of Exploration Geophysicists ; 2023
    In:  GEOPHYSICS Vol. 88, No. 3 ( 2023-05-01), p. E91-E105
    Details
    In: GEOPHYSICS, Society of Exploration Geophysicists, Vol. 88, No. 3 ( 2023-05-01), p. E91-E105
    Abstract: The controlled-source electromagnetic (CSEM) forward modeling with vector and scalar potentials could provide insights into the inductive and galvanic effects. We have developed a finite-element (FE) forward modeling algorithm for the CSEM with vector and scalar potentials. The first-order vector and nodal shape functions are used for the vector and scalar potentials, respectively. To mitigate the null space and thus the nonuniqueness of the potentials caused by the curl operator, we introduce a new preconditioner constructed from an incomplete Cholesky decomposition with zero fill-ins of the Laplacian approximation to the resulted matrix after the FE discretization. We implemented a preconditioned quasiminimal residual method to iteratively solve the resulting linear system with the new preconditioner. We first verified the accuracy of the algorithm through comparison against the analytic solutions obtained for a three-layered earth model. The new iterative modeling algorithm converges much faster compared with the modeling algorithm with the preconditioner constructed from the original stiffness matrix. The efficiency and accuracy are further illustrated by comparison with the modeling scheme based on coupled potentials with explicit enforcement of the Coulomb gauge condition for the vector potential by modeling three 3D models. In addition, the accuracy of our algorithm is demonstrated via a comparison of our numerical solution for a 3D model with the numerical solution obtained from the integral equation method. We also present the inductive and galvanic components of the horizontal secondary electric field for numerical solutions obtained with direct and iterative solvers. The numerical test demonstrated that, aside from the efficiency improved with the new iterative solver, the unstable and nonuniqueness problem for the potentials is eliminated by the new preconditioner with the Laplacian approximation involved.
    Type of Medium: Online Resource
    ISSN: 0016-8033 , 1942-2156
    URL: Article
    DOI: 10.1190/geo2022-0389.1
    RVK:
    UA 4068.4
    Language: English
    Publisher: Society of Exploration Geophysicists
    Publication Date: 2023
    detail.hit.zdb_id: 2033021-2
    detail.hit.zdb_id: 2184-2
    SSG: 16,13
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  • 6
    Online Resource
    Online Resource
    Long-Term Corn–Soybean Rotation and Soil Fertilization: Impacts on Yield and Agronomic Traits
    MDPI AG ; 2022
    In:  Agronomy Vol. 12, No. 10 ( 2022-10-18), p. 2554-
    Details
    In: Agronomy, MDPI AG, Vol. 12, No. 10 ( 2022-10-18), p. 2554-
    Abstract: Although crop rotations have been widely shown as an effective approach for improving yield or soil quality in the long term, the relationship between crop rotations and quality traits of crop or biochar-based fertilization is still unclear. To address this, we conducted a long-term field experiment in the Heilongjiang province of China to investigate the effects of crop rotation and biochar-based fertilizer application on the crop yield, soil parameters, crop quality, and agronomic traits in 2014–2020. The effects of rotations on crop production and soil properties were evaluated with the average yield variability during the 7 years of this study. Our results showed that the diversified corn and soybean rotations had a significant positive effect on average crop yield compared with their monocultures. The corn yield was enhanced by 0.6 Mg ha−1 (5.4%) in the corn–soybean–corn (CSC) crop sequence compared with monoculture corn. Similarly, soybean yield was enhanced by 0.21 Mg ha−1 (9.7%) in the soybean–corn–corn (SCC) crop sequence compared with monoculture soybean. However, a negative effect of crop rotations was detected on the protein content of soybean compared with the monoculture soybean, while a positive effect was detected on oil content. Additionally, no differences were detected in crop yield between biochar-based fertilization and mineral fertilization treatments, but a significant positive effect of biochar-based fertilization was observed for any crop on both protein and oil content. A significant effect of crop rotation was found on the percentage of total soil N (TN), available soil N (AN), and available soil K (AK) content. The SSS crop sequence treatment illustrated the highest TN values at 0.18%. The CCC crop sequence treatment increased AN and AK content by 9.1% and 7.8%, respectively, compared with SSS (p 〈 0.05). We conclude that crop rotations increase crop yield and biochar-based fertilizer application, improving crop quality traits in the long term. Thus, the addition of biochar-based fertilizer could efficiently enhance the yield and quality of crop in the rotation cropping system. The findings of this study may provide useful information for designing sustainable cropping systems based on rotations.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    URL: Article
    DOI: 10.3390/agronomy12102554
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2607043-1
    SSG: 23
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  • 7
    Online Resource
    Online Resource
    DataSheet_4.xlsx
    Frontiers Media SA ; 2021
    In:  Frontiers in Immunology Vol. 12 ( 2021-12-15)
    Details
    In: Frontiers in Immunology, Frontiers Media SA, Vol. 12 ( 2021-12-15)
    Abstract: Early weaning piglet is frequently accompanied by severe enteric inflammatory responses and microbiota dysbiosis. The links between the gut microbiome and the etiology of gut inflammation are not fully understood. The study is aimed to investigate the potential molecular mechanisms mediating inflammatory reactivity following early weaning, and to find whether these changes are correlated with gut microbiota and metabolite signatures by comparison between suckling piglets (SPs) and weaning piglets (WPs). Histopathology analysis showed a severe inflammatory response and the disruption of epithelial barrier function. Early weaning resulted in reduced autophagy indicated as the suppression of autophagic flux, whereas induced the TLR4/P38MAPK/IL-1β-mediated apoptotic pathway, as well as activation of the IL-1β precursor. The alpha-diversity and microbial composition were changed in WPs, such as the decreased abundances of Bifidobacterium , Bacteroides , Bacillus , Lactobacillus , and Ruminococcus . Microbial co-concurrence analysis revealed that early weaning significantly decreased network complexity, including network size, degree, average clustering coefficient and number of keystone species, as compared with the SP group. Differentially abundant metabolites were mainly associated with amino acid and purine metabolism. Strong correlations were detected between discrepant microbial taxa and multiple inflammatory parameters. In conclusion, we found that dysregulations of autophagy and apoptosis pathway were involved in colon inflammation during weaned period, which may result from gut microbiota dysbiosis. This study may provide possible intervention modalities for preventing or treating post-weaning infections through maintaining gut microbial ecosystem integrity.
    Type of Medium: Online Resource
    ISSN: 1664-3224
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3389/fimmu.2021.804870
    DOI: 10.3389/fimmu.2021.804870.s001
    DOI: 10.3389/fimmu.2021.804870.s002
    DOI: 10.3389/fimmu.2021.804870.s003
    DOI: 10.3389/fimmu.2021.804870.s004
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
    detail.hit.zdb_id: 2606827-8
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  • 8
    Online Resource
    Online Resource
    Development of a Doppler-broadened NICE-OHMS system for trace gas detection based on a single sideband phase modulator
    Optica Publishing Group ; 2021
    In:  Optics Express Vol. 29, No. 26 ( 2021-12-20), p. 42411-
    Details
    In: Optics Express, Optica Publishing Group, Vol. 29, No. 26 ( 2021-12-20), p. 42411-
    Abstract: To expand the applicability of noise-immune cavity-enhanced optical heterodyne molecular spectrometer (NICE-OHMS), a universal system incorporating a fiber-coupled single-sideband modulator (f-SSM) for control of the laser frequency has been developed. A homemade PID servo mainly composed of two integrators has been designed, resulting in a locking bandwidth of 170 kHz and a continuous tuning range of 2.2 GHz. The system exhibits a noise-equivalent Doppler-broadened absorption limit of 8.0×10 −14 cm −1 for an integration time of 64 s. Since the f-SSM is the sole external frequency actuator, this opens up for NICE-OHMS based on a multitude of laser systems.
    Type of Medium: Online Resource
    ISSN: 1094-4087
    URL: Article
    DOI: 10.1364/OE.437893
    Language: English
    Publisher: Optica Publishing Group
    Publication Date: 2021
    detail.hit.zdb_id: 1491859-6
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  • 9
    Online Resource
    Online Resource
    A novel methodology to directly pre-determine the relative wavelength response of DFB laser in wavelength modulation spectroscopy
    Optica Publishing Group ; 2019
    In:  Optics Express Vol. 27, No. 2 ( 2019-01-21), p. 1249-
    Details
    In: Optics Express, Optica Publishing Group, Vol. 27, No. 2 ( 2019-01-21), p. 1249-
    Type of Medium: Online Resource
    ISSN: 1094-4087
    URL: Article
    DOI: 10.1364/OE.27.001249
    Language: English
    Publisher: Optica Publishing Group
    Publication Date: 2019
    detail.hit.zdb_id: 1491859-6
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  • 10
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    Online Resource
    Towards the development of risk-constrained optimal bidding strategies for generation companies in electricity markets
    Elsevier BV ; 2005
    In:  Electric Power Systems Research Vol. 73, No. 3 ( 2005-3), p. 305-312
    Details
    In: Electric Power Systems Research, Elsevier BV, Vol. 73, No. 3 ( 2005-3), p. 305-312
    Type of Medium: Online Resource
    ISSN: 0378-7796
    URL: Article
    DOI: 10.1016/j.epsr.2004.07.004
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2005
    detail.hit.zdb_id: 1502242-0
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