In:
Applied Mechanics and Materials, Trans Tech Publications, Ltd., Vol. 195-196 ( 2012-8), p. 452-458
Abstract:
Construction of Eukaryotic recombinant expression Vector pCI-ORF5-ORF6/ pCI-ORF5-ORF6B-Hsp70 Containing Hsp70 and PRRSV GP5/M (encoded by ORF5 and ORF6 genes), and to study its immune effect. After ifentified by enzyme analysis and nucleotide sequencing test,the repression vector plasmid was transfected into COS-7 cells. The transient expression protein was detected by indirect immunofluorescence assay (IFA) and Western-blotting. The immunogenicities of this DNA vaccine constructs were firstly investigated in a mouse moder. IFN-γ, IL-4 of cytokine, and the spleen T-lymphocyte subgroup quantity (CD4+/CD8+) were detected. The result shows that the recombinant plasmid pCI-ORF5-ORF6-Hsp70 could induce higher response of cellular immune responses and specific hummune responses in mouse, providing the clinical basic data and theoretical basis for success of the DNA vaccine development.
Type of Medium:
Online Resource
ISSN:
1662-7482
DOI:
10.4028/www.scientific.net/AMM.195-196
DOI:
10.4028/www.scientific.net/AMM.195-196.452
Language:
Unknown
Publisher:
Trans Tech Publications, Ltd.
Publication Date:
2012
detail.hit.zdb_id:
2251882-4
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