In:
The Plant Journal, Wiley, Vol. 80, No. 6 ( 2014-12), p. 1072-1084
Abstract:
Microbe‐associated molecular pattern ( MAMP )‐triggered immunity plays critical roles in the basal resistance defense response in plants. Chitin and peptidoglycan ( PGN ) are major molecular patterns for fungi and bacteria, respectively. Two rice ( Oryza sativa ) lysin motif‐containing proteins, Os LYP 4 and Os LYP 6, function as receptors that sense bacterial PGN and fungal chitin. These membrane receptors, which lack intracellular kinase domains, likely contain another component for transmembrane immune signal transduction. Here, we demonstrate that the rice LysM receptor‐like kinase Os CERK 1, a key component of the chitin elicitor signaling pathway, also plays an important role in PGN ‐triggered immunity in rice. Silencing of Os CERK 1 suppressed PGN ‐induced (and chitin‐induced) immunity responses, including reactive oxygen species generation, defense gene expression, and callose deposition, indicating that Os CERK 1 is essential for both PGN and chitin signaling initiated by Os LYP 4 and Os LYP 6. Os LYP 4 associated with Os LYP 6 and the rice chitin receptor chitin oligosaccharide elicitor‐binding protein ( CEB iP) in the absence of PGN or chitin, and treatment with PGN or chitin led to their disassociation in vivo . Os CERK 1 associated with Os LYP 4 or Os LYP 6 when induced by PGN but it associated with Os LYP 4, Os LYP 6, or CEB iP under chitin treatment, suggesting the presence of different patterns of ligand‐induced heterooligomeric receptor complexes. Furthermore, the receptor‐like cytoplasmic kinase Os RLCK 176 functions downstream of Os CERK 1 in the PGN and chitin signaling pathways, suggesting that these MAMP s share overlapping intracellular signaling components. Therefore, Os CERK 1 plays dual roles in PGN and chitin signaling in rice innate immunity and as an adaptor involved in signal transduction at the plasma membrane in conjunction with Os LYP 4 and Os LYP 6.
Type of Medium:
Online Resource
ISSN:
0960-7412
,
1365-313X
DOI:
10.1111/tpj.2014.80.issue-6
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2020961-7
SSG:
12
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