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  • SAGE Publications  (5)
  • Lin, W.  (5)
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  • SAGE Publications  (5)
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  • 1
    Online Resource
    Online Resource
    CBD Promotes Oral Ulcer Healing via Inhibiting CMPK2-Mediated Inflammasome
    SAGE Publications ; 2022
    In:  Journal of Dental Research Vol. 101, No. 2 ( 2022-02), p. 206-215
    Details
    In: Journal of Dental Research, SAGE Publications, Vol. 101, No. 2 ( 2022-02), p. 206-215
    Abstract: Oral ulcer is a common oral inflammatory lesion accompanied by severe pain but with few effective treatments. Cannabidiol (CBD) is recently emerging for its therapeutic potential in a range of diseases, including inflammatory conditions and cancers. Here we show that CBD oral spray on acid- or trauma-induced oral ulcers on mice tongue inhibits inflammation, relieves pain, and accelerates lesion closure. Notably, the enrichment of genes associated with the NOD, LRR, and NLRP3 pyrin domain–containing protein 3 (NLRP3) inflammasome pathway is downregulated after CBD treatment. The expression of cleaved-gasdermin D (GSDMD) and the percentage of pyroptotic cells are reduced as well. In addition, CBD decreases the expression of cytidine/uridine monophosphate kinase 2 (CMPK2), which subsequently inhibits the generation of oxidized mitochondria DNA and suppresses inflammasome activation. These immunomodulating effects of CBD are mostly blocked by peroxisome proliferator activated receptor γ (PPARγ) antagonist and partially antagonized by CB 1 receptor antagonist. Our results demonstrate that CBD accelerates oral ulcer healing by inhibiting CMPK2-mediated NLRP3 inflammasome activation and pyroptosis, which are mediated mostly by PPARγ in the nucleus and partially by CB 1 in the plasma membrane.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    URL: Article
    DOI: 10.1177/00220345211024528
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
    detail.hit.zdb_id: 2057074-0
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  • 2
    Online Resource
    Online Resource
    Single-Cell Transcriptomic Atlas of Gingival Mucosa in Type 2 Diabetes
    SAGE Publications ; 2022
    In:  Journal of Dental Research Vol. 101, No. 13 ( 2022-12), p. 1654-1664
    Details
    In: Journal of Dental Research, SAGE Publications, Vol. 101, No. 13 ( 2022-12), p. 1654-1664
    Abstract: The oral gingival barrier is a constantly stimulated and dynamic environment where homeostasis is often disrupted, resulting in inflammatory periodontal diseases. Type 2 diabetes (T2D) has been reported to be associated with gingival barrier dysfunction, but the effect and underlying mechanism are inconclusive. Herein, we performed single-cell RNA sequencing (scRNA-seq) of gingiva from leptin receptor-deficient mice ( db/db) to examine the gingival heterogeneity in the context of T2D. Periodontal health of control mice is characterized by populations of Krt14 + -expressing epithelial cells and Col1a1 + -fibroblasts mediating immune homeostasis primarily through the enrichment of innate lymphoid cells. The db/db gingiva exhibited decreased epithelial/stromal ratio and dysfunctional barrier. We further observed stromal, particularly fibroblast immune hyperresponsiveness, linked to the recruitment of myeloid-derived cells at the db/db gingiva. Both scRNA-seq and histological analysis suggested the inflammatory signaling between fibroblasts and neutrophils as a potential driver of diabetes-induced periodontal damage. Notably, the “immune-like” stromal cells were wired toward the induction of gingival IL-17A hyperresponsiveness in db/db mice. Our work reveals that the “immune-like” fibroblasts with transcriptional diversity are involved in the innate immune homeostasis at the diabetic gingiva. It highlights a potentially significant role of these cell types in its pathogenesis.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    URL: Article
    DOI: 10.1177/00220345221092752
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
    detail.hit.zdb_id: 2057074-0
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  • 3
    Online Resource
    Online Resource
    Seismic Behavior of Long-Span Connected Structures under Multi-Supported and Multi-Dimensional Earthquake Excitations
    SAGE Publications ; 2013
    In:  Advances in Structural Engineering Vol. 16, No. 9 ( 2013-09), p. 1579-1586
    Details
    In: Advances in Structural Engineering, SAGE Publications, Vol. 16, No. 9 ( 2013-09), p. 1579-1586
    Abstract: Seismic behaviors of long-span connected structures under multi-dimensional and earthquake excitation while considering wave passage effect are investigated through both numerical simulation and a shaking table test on a scaled model. It is found that seismic responses will be increased significantly if multi-dimensional earthquake excitation is considered; and wave-passage effect has diverse influence on different elements. Also special attentions should be paid to those elements near connections between the main tower and the corridor which inertial forces may change a lot when subjected to Traveling wave excitation with different apparent velocities. Moreover, the vertical acceleration responses of the corridor can be increased significantly due to wave passage effect. Therefore, it is necessary for aseismic design of long-span connected structure to take into account multi-dimensional earthquake components and multi-support earthquake excitations with different apparent velocities.
    Type of Medium: Online Resource
    ISSN: 1369-4332 , 2048-4011
    URL: Article
    DOI: 10.1260/1369-4332.16.9.1579
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2013
    detail.hit.zdb_id: 2026561-X
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  • 4
    Online Resource
    Online Resource
    Transcriptomic Mapping of Human Parotid Gland at Single-Cell Resolution
    SAGE Publications ; 2022
    In:  Journal of Dental Research Vol. 101, No. 8 ( 2022-07), p. 972-982
    Details
    In: Journal of Dental Research, SAGE Publications, Vol. 101, No. 8 ( 2022-07), p. 972-982
    Abstract: As the largest salivary gland in oral cavity, the parotid gland plays an important role in initial digesting and lubricating food. The abnormal secretory function of the parotid gland can lead to dental caries and oral mucosal inflammation. In recent years, single-cell RNA sequencing (scRNA-seq) has been used to explore the heterogeneity and diversity of cells in various organs and tissues. However, the transcription profile of the human parotid gland at single-cell resolution has not been reported yet. In this study, we constructed the cell atlas of human parotid gland using the 10× Genomics platform. Characteristic gene analysis identified the biological functions of serous acinar cell populations in secreting digestive enzymes and antibacterial proteins. We revealed the specificity and similarity of the parotid gland compared to other digestive glands through comparative analyses of other published scRNA-seq data sets. We also identified the cell-specific expression of hub genes for Sjögren syndrome in the human parotid gland by integrating the results of genome-wide association studies and bulk RNA-seq, which highlighted the importance of immune cell dysfunction in parotid Sjögren syndrome pathogenesis.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    URL: Article
    DOI: 10.1177/00220345221076069
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
    detail.hit.zdb_id: 2057074-0
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  • 5
    Online Resource
    Online Resource
    Gnas Loss Causes Chondrocyte Fate Conversion in Cranial Suture Formation
    SAGE Publications ; 2022
    In:  Journal of Dental Research Vol. 101, No. 8 ( 2022-07), p. 931-941
    Details
    In: Journal of Dental Research, SAGE Publications, Vol. 101, No. 8 ( 2022-07), p. 931-941
    Abstract: Calvaria development is distinct from limb formation. Craniosynostosis is a skull deformity characterized by premature cranial suture fusion due to the loss of the GNAS gene and, consequently, its encoded protein Gα s . This birth defect requires surgery, with potential lethal consequences. So far, hardly any early-stage nonsurgical interventions for GNAS loss-related craniosynostosis are available. Here, we investigated the role of the Gnas gene in mice in guarding the distinctiveness of intramembranous ossification and how loss of Gnas triggered endochondral-like ossification within the cranial sutures. Single-cell RNA sequencing (scRNA-seq) of normal neonatal mice cranial suture chondrocytes showed a Hedgehog (Hh) inactivation pattern, which was associated with Gα s signaling activation. Loss of Gnas evoked chondrocyte-to-osteoblast fate conversion and resulted in cartilage heterotopic ossification (HO) within cranial sutures and fontanels of the mouse model, leading to a skull deformity resembling craniosynostosis in patients with loss of GNAS. Activation of ectopic Hh signaling within cranial chondrocytes stimulated the conversion of cell identity through a hypertrophy-like stage, which shared features of endochondral ossification in vivo. Reduction of Gli transcription activity by crossing with a loss-of-function Gli2 allele or injecting GLI1/2 antagonist hindered the progression of cartilage HO in neonatal stage mice. Our study uncovered the role of Gα s in maintaining cranial chondrocyte identity during neonatal calvaria development in mice and how reduction of Hh signaling could be a nonsurgical intervention to reduce skull deformity in craniosynostosis due to loss of GNAS.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    URL: Article
    DOI: 10.1177/00220345221075215
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
    detail.hit.zdb_id: 2057074-0
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