In:
Journal of Basic Microbiology, Wiley, Vol. 60, No. 1 ( 2020-01), p. 72-81
Abstract:
Salmonella Enteritidis is an important foodborne pathogen that can infect a wide range of animal species including human beings, resulting in great losses to commercial husbandry and human health. CirA is an outer membrane receptor involved in iron uptake and colicin1A/B‐mediated competitive killing. Although iron uptake is crucial to bacterial virulence, limited literature is available about the role of CirA in infection. In the present work, we aimed to evaluate the role of CirA during S. Enteritidis infection. For this purpose, we generated a CirA‐deficient mutant of the S. Enteritidis strain C50336 and examined its biological characteristics. The results showed that cirA gene inactivation caused sharply decreased biofilm formation and apparently impaired antibiotic resistance. Furthermore, the cirA gene deletion mutant showed markedly reduced adhesion and invasion to human epithelial cell line Caco‐2 cells and decreased proliferation in mouse macrophage cell line RAW264.7 cells. Moreover, attenuated virulence was determined by a mouse model, with an LD 50 increase of approximately 1,000‐fold. These data indicated that CirA plays critical roles in the S. Enteritidis infection process.
Type of Medium:
Online Resource
ISSN:
0233-111X
,
1521-4028
DOI:
10.1002/jobm.201900418
Language:
English
Publisher:
Wiley
Publication Date:
2020
detail.hit.zdb_id:
1480967-9
detail.hit.zdb_id:
632513-0
detail.hit.zdb_id:
203025-1
SSG:
12
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