In:
Frontiers in Immunology, Frontiers Media SA, Vol. 13 ( 2022-7-6)
Abstract:
Understanding immune cell phenotypes in the tumor microenvironment (TME) is essential for explaining and predicting progression of non-small cell lung cancer (NSCLC) and its response to immunotherapy. Here we describe the single-cell transcriptomics of CD45 + immune cells from tumors, normal tissues and blood of NSCLC patients. We identified three clusters of immune cells exerting immunosuppressive effects: CD8 + T cells with exhausted phenotype, tumor-associated macrophages (TAMs) with a pro-inflammatory M2 phenotype, and regulatory B cells (B regs) with tumor-promoting characteristics. We identified genes that may be mediating T cell phenotypes, including the transcription factors ONECUT2 and ETV4 in exhausted CD8 + T cells, TIGIT and CTL4 high expression in regulatory T cells. Our results highlight the heterogeneity of CD45 + immune cells in the TME and provide testable hypotheses about the cell types and genes that define the TME.
Type of Medium:
Online Resource
ISSN:
1664-3224
DOI:
10.3389/fimmu.2022.854724
DOI:
10.3389/fimmu.2022.854724.s001
DOI:
10.3389/fimmu.2022.854724.s002
DOI:
10.3389/fimmu.2022.854724.s003
DOI:
10.3389/fimmu.2022.854724.s004
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2606827-8
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