In:
Mediators of Inflammation, Hindawi Limited, Vol. 2013 ( 2013), p. 1-12
Abstract:
The aim of this study was to investigate the anti-inflammatory effect of IL-21 on LPS-induced mouse peritoneal macrophages. The results showed that IL-21 significantly inhibited LPS-induced mRNA expression of IL-1 β , TNF- α , and IL-6 in macrophages, but not of IFN- γ , IL-10, CCL5, or CXCL2. ELISA analysis showed that IL-21 also suppressed LPS-induced production of TNF- α and IL-6 in culture supernatants. Western blot analysis showed that IL-21 clearly inhibited ERK and I κ B α phosphorylation and NF- κ B translocation in LPS-stimulated macrophages, but it increased STAT3 phosphorylation. Flow cytometric and Western blot analysis showed that IL-21 decreased M1 macrophages surface markers expression of CD86, iNOS, and TLR4 in LPS-stimulated cells. All results suggested that IL-21 decreases IL-6 and TNF- α production via inhibiting the phosphorylation of ERK and translocation of NF- κ B and promotes a shift from the M1 to M2 macrophage phenotype by decreasing the expression of CD86, iNOS, and TLR4 and by increasing STAT3 phosphorylation in LPS-stimulated cells.
Type of Medium:
Online Resource
ISSN:
0962-9351
,
1466-1861
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2013
detail.hit.zdb_id:
2008065-7
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