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  • MDPI AG  (38)
  • Li, Yan  (38)
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  • MDPI AG  (38)
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  • 1
    Online Resource
    Online Resource
    Pre-Administration of Berberine Exerts Chemopreventive Effects in AOM/DSS-Induced Colitis-Associated Carcinogenesis Mice via Modulating Inflammation and Intestinal Microbiota
    MDPI AG ; 2022
    In:  Nutrients Vol. 14, No. 4 ( 2022-02-09), p. 726-
    Details
    In: Nutrients, MDPI AG, Vol. 14, No. 4 ( 2022-02-09), p. 726-
    Abstract: Inflammatory activation and intestinal flora imbalance play an essential role in the development and progression of colorectal cancer (CRC). Berberine (BBR) has attracted great attention in recent years due to its heath-related benefits in inflammatory disorders and tumors, but the intricate mechanisms have not been fully elucidated. In this study, the effects and the mechanism of BBR on colon cancer were investigated in an azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colitis-associated carcinogenesis mice model. Our results showed that pre-administration of BBR showed a decrease in weight loss, disease activity index (DAI) score, and the number of colon tumors in mice, compared with the model group. The evidence from pathological examination indicated that the malignancy of intestinal tumors was ameliorated after pre-administration of BBR. Additionally, pre-administration with BBR suppressed the expression of pro-inflammatory factors (interleukin (IL)-6, IL-1β, cyclooxygenase (COX)-2 and tumor necrosis factor (TNF)-α) and the cell-proliferation marker Ki67, while expression of the tight junction proteins (ZO-1 and occludin) were increased in colon tissue. Moreover, the levels of critical pathway proteins involved in the inflammatory process (p-STAT3 and p-JNK) and cell cycle regulation molecules (β-catenin, c-Myc and CylinD1) exhibited lower expression levels. Besides, 16S rRNA sequence analysis indicated that pre-administration of BBR increased the ratio of Firmicutes/Bacteroidetes (F:M) and the relative abundance of potentially beneficial bacteria, while the abundance of cancer-related bacteria was decreased. Gavage with Lactobacillus rhamnosus can improve the anti-tumor effect of BBR. Overall, pre-administration of BBR exerts preventive effects in colon carcinogenesis, and the mechanisms underlying these effects are correlated with the inhibition of inflammation and tumor proliferation and the maintenance of intestinal homeostasis.
    Type of Medium: Online Resource
    ISSN: 2072-6643
    URL: Article
    DOI: 10.3390/nu14040726
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2518386-2
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  • 2
    Online Resource
    Online Resource
    Network Pharmacology Exploration Reveals Anti-Apoptosis as a Common Therapeutic Mechanism for Non-Alcoholic Fatty Liver Disease Treated with Blueberry Leaf Polyphenols
    MDPI AG ; 2021
    In:  Nutrients Vol. 13, No. 11 ( 2021-11-13), p. 4060-
    Details
    In: Nutrients, MDPI AG, Vol. 13, No. 11 ( 2021-11-13), p. 4060-
    Abstract: Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease characterized by excessive fat accumulation in the liver. The aim of this study is to elucidate the multi-target mechanism of polyphenols in blueberry leaves (PBL) on NAFLD by network pharmacology and to validate its results via biological experiments. Twenty constituents in PBL were preliminarily determined by liquid chromatography-tandem mass spectrometry. Subsequently, 141 predicted drug targets and 1226 targets associated with NAFLD were retrieved from public databases, respectively. The herb-compound-target network and the target protein–protein interaction network (PPI) were established through Cytoscape software, and four compounds and 53 corresponding targets were identified. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment were performed to explore the biological processes of the predicted genes. The results of cell experiments demonstrated that PBL could significantly improve the viability of the NAFLD cell model, and the protein expressions of caspase-3 and Bcl-2 were consistent with the expected mechanism of action of PBL. Those results systematically revealed that the multi-target mechanism of PBL against NAFLD was related to the apoptosis pathway, which could bring deeper reflections into the hepatoprotective effect of PBL.
    Type of Medium: Online Resource
    ISSN: 2072-6643
    URL: Article
    DOI: 10.3390/nu13114060
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2518386-2
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  • 3
    Online Resource
    Online Resource
    Immune Responses Regulated by Key Periodontal Bacteria in Germ-Free Mice
    MDPI AG ; 2022
    In:  Pathogens Vol. 11, No. 5 ( 2022-04-26), p. 513-
    Details
    In: Pathogens, MDPI AG, Vol. 11, No. 5 ( 2022-04-26), p. 513-
    Abstract: The immune dysregulation induced by periodontal bacteria has important roles in the development of periodontitis. However, the role of key periodontal bacteria in local and systemic immunity has not been comprehensively studied. Herein, to explore immunoregulation maps of key periodontal bacteria, a mono-colonized germ-free mice model with P. gingivalis, F. nucleatum, and T. denticola for two weeks was designed in this study. The alveolar bone loss was determined by micro-CT. A total of 14 types of innate and adaptive immune cells of the gingiva, spleen, and colon were detected by multi-color flow cytometry. P. gingivalis induced the strongest innate immune response in gingiva and mononuclear phagocytes (MNPs) changed most significantly, compared to F. nucleatum and T. denticola. Immune dysregulation of the colon was widely induced by F. nucleatum. T. denticola mainly induced immune disorder in spleen. ILC3s, Tregs, CD11B+ dendritic cells s, MNPs, macrophages, and plasmacytoid dendritic cells were the main types in response to key periodontal bacteria. However, the alveolar bone loss was not induced by key periodontal bacteria. In conclusion, the overall immunoregulation of monomicrobial stimuli to decipher the complexities of periodontitis was provided in this study. P. gingivalis, F. nucleatum, and T. denticola have different effects on local and systemic immunity in gingiva, colon, and spleen of germ-free mice.
    Type of Medium: Online Resource
    ISSN: 2076-0817
    URL: Article
    DOI: 10.3390/pathogens11050513
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2695572-6
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  • 4
    Online Resource
    Online Resource
    Assessing and Prioritising Delay Factors of Prefabricated Concrete Building Projects in China
    MDPI AG ; 2018
    In:  Applied Sciences Vol. 8, No. 11 ( 2018-11-21), p. 2324-
    Details
    In: Applied Sciences, MDPI AG, Vol. 8, No. 11 ( 2018-11-21), p. 2324-
    Abstract: Prefabricated construction has been widely accepted as an alternative to conventional cast-in-situ construction, given its improved performance. However, prefabricated concrete building projects frequently encounter significant delays. It is, therefore, crucial to identify key factors affecting schedule and explore strategies to minimise the schedule delays for prefabricated concrete building projects. This paper adopts the decision-making trial and evaluation laboratory (DEMATEL) model and analytic network process (ANP) method to quantify the cause-and-effect relationships and prioritise the key delay factors in terms of their importance in the Chinese construction industry. The DEMATEL model evaluates the extent to which each factor impacts other factors. The quantified extents are then converted into a prioritisation matrix through ANP. The delay factors of prefabricated construction projects are selected and categorised based on a literature review and an expert interview. Questionnaires are then implemented to collect the data. The results reveal that the issue of inefficient structural connections for prefabricated components is found to be the most significant factor and most easily affected by other delay factors. This research also suggests prioritising major delay factors, such as ‘lack of communication among participants’ and ‘low productivity’, in the Chinese construction industry during scheduling control. Overall, this research contributes an assessment framework for decision making in the scheduling management of prefabricated construction.
    Type of Medium: Online Resource
    ISSN: 2076-3417
    URL: Article
    DOI: 10.3390/app8112324
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2704225-X
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  • 5
    Online Resource
    Online Resource
    Cryopreserved Human Natural Killer Cells Exhibit Potent Antitumor Efficacy against Orthotopic Pancreatic Cancer through Efficient Tumor-Homing and Cytolytic Ability
    MDPI AG ; 2019
    In:  Cancers Vol. 11, No. 7 ( 2019-07-09), p. 966-
    Details
    In: Cancers, MDPI AG, Vol. 11, No. 7 ( 2019-07-09), p. 966-
    Abstract: Pancreatic cancer is known to be highly aggressive, and desmoplasia-induced accumulation of extracellular matrix (ECM), which is a hallmark of many pancreatic cancers, severely restricts the therapeutic efficacy of both immunotherapeutics and conventional chemotherapeutics due to the ECM functioning as a major physical barrier against permeation and penetration. In the case of cell-based immunotherapeutics, there are several other bottlenecks preventing translation into clinical use due to their biological nature; for example, poor availability of cell therapeutic in a readily usable form due to difficulties in production, handling, shipping, and storage. To address these challenges, we have isolated allogeneic natural killer (NK) cells from healthy donors and expanded them in vitro to generate cryopreserved stocks. These cryopreserved NK cells were thawed to evaluate their therapeutic efficacy against desmoplastic pancreatic tumors, ultimately aiming to develop a readily accessible and mass-producible off-the-shelf cell-based immunotherapeutic. The cultured NK cells post-thawing retained highly pure populations of activated NK cells that expressed various activating receptors and a chemokine receptor. Furthermore, systemic administration of NK cells induced greater in vivo tumor growth suppression when compared with gemcitabine, which is the standard chemotherapeutic used for pancreatic cancer treatment. The potent antitumor effect of NK cells was mediated by efficient tumor-homing ability and infiltration into desmoplastic tumor tissues. Moreover, the infiltration of NK cells led to strong induction of apoptosis, elevated expression of the antitumor cytokine interferon (IFN)-γ, and inhibited expression of the immunosuppressive transforming growth factor (TGF)-β in tumor tissues. Expanded and cryopreserved NK cells are strong candidates for future cell-mediated systemic immunotherapy against pancreatic cancer.
    Type of Medium: Online Resource
    ISSN: 2072-6694
    URL: Article
    DOI: 10.3390/cancers11070966
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2527080-1
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  • 6
    Online Resource
    Online Resource
    Plastidial Phosphoglucomutase (pPGM) Overexpression Increases the Starch Content of Transgenic Sweet Potato Storage Roots
    MDPI AG ; 2022
    In:  Genes Vol. 13, No. 12 ( 2022-11-28), p. 2234-
    Details
    In: Genes, MDPI AG, Vol. 13, No. 12 ( 2022-11-28), p. 2234-
    Abstract: Sweet potato (Ipomoea batatas), an important root crop, has storage roots rich in starch that are edible and serve as a raw material in bioenergy production. Increasing the storage-root starch contents is a key sweet potato breeding goal. Phosphoglucomutase (PGM) is the catalytic enzyme for the interconversion of glucose-6-phosphate and glucose-1-phosphate, precursors in the plant starch synthetic pathway. Plant PGMs have plastidial and cytosolic isoforms, based on their subcellular localization. Here, IbpPGM, containing 22 exons and 21 introns, was cloned from the sweet potato line Xu 781. This gene was highly expressed in the storage roots and leaves, and its expression was induced by exogenous sucrose treatments. The mature IbpPGM protein was successfully expressed in Escherichia coli when a 73-aa chloroplastic transit peptide detected in the N-terminus was excised. The subcellular localization confirmed that IbpPGM was localized to the chloroplasts. The low-starch sweet potato cultivar Lizixiang IbpPGM-overexpression lines showed significantly increased starch, glucose, and fructose levels but a decreased sucrose level. Additionally, the expression levels of the starch synthetic pathway genes in the storage roots were up-regulated to different extents. Thus, IbpPGM significantly increased the starch content of the sweet potato storage roots, which makes it a candidate gene for the genetic engineering of the sweet potato.
    Type of Medium: Online Resource
    ISSN: 2073-4425
    URL: Article
    DOI: 10.3390/genes13122234
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2527218-4
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  • 7
    Online Resource
    Online Resource
    A Review of Tunable Orbital Angular Momentum Modes in Fiber: Principle and Generation
    MDPI AG ; 2019
    In:  Applied Sciences Vol. 9, No. 12 ( 2019-06-13), p. 2408-
    Details
    In: Applied Sciences, MDPI AG, Vol. 9, No. 12 ( 2019-06-13), p. 2408-
    Abstract: Orbital angular momentum (OAM) beams, a new fundamental degree of freedom, have excited a great diversity of interest due to a variety of emerging applications. The scalability of OAM has always been a topic of discussion because it plays an important role in many applications, such as expanding to large capacity and adjusting the trapped particle rotation speed. Thus, the generation of arbitrary tunable OAM mode has been paid increasing attention. In this paper, the basic concepts of classical OAM modes are introduced firstly. Then, the tunable OAM modes are categorized into three types according to the orbital angular momentums and polarization states of mode carrying. In order to understand the OAM evolution of a mode intuitively, three kinds of Poincaré spheres (PSs) are introduced to represent the three kinds of tunable OAM modes. Numerous methods generating tunable OAM modes can be roughly divided into two types: spatial and fiber-based generation methods. The principles of fiber-based generation methods are interpreted by introducing two mode bases (linearly-polarized modes and vector modes) of the fiber. Finally, the strengths and weaknesses of each generation method are pointed out and the key challenges for tunable OAM modes are discussed.
    Type of Medium: Online Resource
    ISSN: 2076-3417
    URL: Article
    DOI: 10.3390/app9122408
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2704225-X
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  • 8
    Online Resource
    Online Resource
    Post-FEC Performance of Pilot-Aided Carrier Phase Estimation over Cycle Slip
    MDPI AG ; 2019
    In:  Applied Sciences Vol. 9, No. 13 ( 2019-07-08), p. 2749-
    Details
    In: Applied Sciences, MDPI AG, Vol. 9, No. 13 ( 2019-07-08), p. 2749-
    Abstract: The POST-forward error correction (FEC) bit error rate (BER) performance and the cycle-slip (CS) probability of the carrier phase estimation (CPE) scheme based on Viterbi–Viterbi phase estimation (VVPE) algorithm and the VV cascaded by pilot-aided-phase-unwrap (PAPU) algorithm have been experimentally investigated in a 56 Gbit/s quadrature phase-shift keying (QPSK) coherent communication system. Experimental results show that, with 0.78% pilot overhead, the VVPE + PAPU scheme greatly improves the POST-FEC performance degraded by continuous CS, maintaining a low CS probability with less influence of filter length. Comparing with the VVPE scheme, the VVPE + PAPU scheme can respectively obtain about 3.1 dB, 1.3 dB, 0.6 dB PRE-FEC optical signal noise ratio (OSNR) gains at PRE-BER of 1.8 × 10−2. Meanwhile, the VVPE + PAPU scheme respectively achieves about 3 dB, 1 dB, and 0.5 dB POST-FEC OSNR gain and improves the FEC limit from 2.5 × 10−3 to 1.4 × 10−2, from 8.9 × 10−3 to 1.8 × 10−2, and from 1.6 × 10−2 to 1.9 × 10−2 under the CPE filter length of 8, 16, and 20.
    Type of Medium: Online Resource
    ISSN: 2076-3417
    URL: Article
    DOI: 10.3390/app9132749
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2704225-X
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  • 9
    Online Resource
    Online Resource
    In Silico Identification of Structure Requirement for Novel Thiazole and Oxazole Derivatives as Potent Fructose 1,6-Bisphosphatase Inhibitors
    MDPI AG ; 2011
    In:  International Journal of Molecular Sciences Vol. 12, No. 11 ( 2011-11-18), p. 8161-8180
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 12, No. 11 ( 2011-11-18), p. 8161-8180
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms12118161
    Language: English
    Publisher: MDPI AG
    Publication Date: 2011
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 10
    Online Resource
    Online Resource
    Curcumin: Updated Molecular Mechanisms and Intervention Targets in Human Lung Cancer
    MDPI AG ; 2012
    In:  International Journal of Molecular Sciences Vol. 13, No. 3 ( 2012-03-22), p. 3959-3978
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 13, No. 3 ( 2012-03-22), p. 3959-3978
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms13033959
    Language: English
    Publisher: MDPI AG
    Publication Date: 2012
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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