In:
Applied Optics, Optica Publishing Group, Vol. 60, No. 32 ( 2021-11-10), p. 10239-
Abstract:
Airy beam light-sheet illumination can extend the field of view (FOV)
of light-sheet fluorescence microscopy due to the unique propagation properties of non-diffraction and self-acceleration. However, the side
lobes create undesirable out-of-focus background, leading to poor axial resolution and low image contrast. Here, we propose an Airy
complementary beam subtraction (ACBS) method to improve the axial resolution while keeping the extended FOV. By scanning the optimized
designed complementary beam that has two main lobes (TML), the generated complementary light-sheet has almost identical intensity
distribution to that of the planar Airy light-sheet except for the central lobe. Subtraction of the two images acquired by double
exposure respectively using the planar Airy light-sheet and the planar TML light-sheet can effectively suppress the influence of the
out-of-focus background. The axial resolution improves from ∼ 4 µ m to 1.2 µm. The imaging performance
was demonstrated by imaging specimens of aspergillus conidiophores and GFP labeled mouse brain section. The results show that the ACBS method
enables the Airy beam light-sheet fluorescence microscopy to obtain better imaging quality.
Type of Medium:
Online Resource
ISSN:
1559-128X
,
2155-3165
Language:
English
Publisher:
Optica Publishing Group
Publication Date:
2021
detail.hit.zdb_id:
207387-0
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