In:
Journal of Molecular Biology Research, Canadian Center of Science and Education, Vol. 8, No. 1 ( 2018-11-30), p. 167-
Abstract:
As one crucial member of heat shock proteins (HSPs) family, HSP70s play many important roles in a large amount of physiological processes including immune response. However, information regarding HSP70 in ark shell Scapharca broughtonii is still rather limited. Here the full-length cDNA of HSP70 gene (named SbHSP70) of S. broughtonii was identified by using reverse transcription PCR (RT-PCR) and rapid amplification ends (RACE) methods. The SbHSP70 cDNA was 2423 bp in length containing a 5 & prime; untranslated region (UTR) of 131 bp, 3 & prime;-UTR of 330 bp, and an open reading frame (ORF) of 1962 bp which encodes a peptide of 653 amino acids.The multiple alignment and phylogeny analysis showed that the SbHSP70 shared high homology sequence with other mollusk species, and clustered together with gastropods to form a sister group. The mRNA expression profiles of SbHSP70 in tissues of foot, gill, mantle, adductor muscle, haemocytes and hepatopancreas analyzed by quantitative real-time PCR (qRT-PCR) suggested the mRNA transcripts of SbHSP70 distributed in all the examined tissues, and the highest expression level was observed in foot, and a significant difference could be detected between gill and adductor muscle (p & lt;0.05), no significant difference among the gill, mantle and hemocytes (p & gt; 0.05). Its dynamic change during the early stage of larvae showed that it could be transferred from parent and may be involved in some key developmental process. What & rsquo;s more, Vibrio anguillarum challenge resulted in regular change of expression of SbHSP70 mRNA, indicating SbHSP70 actively participated in the immune response process.
Type of Medium:
Online Resource
ISSN:
1925-4318
,
1925-430X
DOI:
10.5539/jmbr.v8n1p167
Language:
Unknown
Publisher:
Canadian Center of Science and Education
Publication Date:
2018
detail.hit.zdb_id:
2649718-9
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