In:
Reproduction in Domestic Animals, Wiley, Vol. 53, No. 5 ( 2018-10), p. 1227-1234
Abstract:
Adipokines play a crucial role in the regulation of energy homeostasis; however, little is known about genetic alterations in this family that may contribute to economic traits in cattle. Therefore, this study conducts transcript profiles, variations and association studies of three major adipokines, leptin ( LEP ) , tumour necrosis factor ( TNF ) and angiopoietin‐like protein 8 ( ANGPTL 8), to evaluate their effects on native Chinese cattle. Using quantitative real‐time PCR , the study revealed that the bovine LEP was expressed primarily in the back and visceral fat, while TNF was predominantly expressed in spleen and ANGPTL 8 was mainly expressed in back fat and liver. Five single nucleotide polymorphisms ( SNP s) including two missense SNP s ( SNP 1: g.12254T 〉 C and SNP 2: g.14177C 〉 T) in LEP , a synonymous SNP ( SNP 3: g.2130A 〉 G) in TNF and two SNP s ( SNP 4: g.629G 〉 A and SNP 5: g.884T 〉 C) in the 5′ UTR of ANGPTL 8 were identified and genotyped in 537 individuals from six Chinese cattle breeds. Bioinformatics analysis revealed that SNP 1 might disrupt the efficient binding of LEP to its receptor, SNP 3 might affect translation efficiency of TNF , and SNP 4 and SNP 5 were likely to affect stability, splicing and nuclear export of ANGPTL 8 mRNA . Consistently, association studies indicated that three SNP s ( SNP 1, SNP 3 and SNP 5) were significantly associated with body weight, heart girth, average daily gain, hip width and body length in 100 Nanyang cattle ( p 〈 0.05). Overall, our results support the view that LEP , TNF and ANGPTL 8 could be used as biomarkers to improve the growth performance in Chinese cattle selection programmes.
Type of Medium:
Online Resource
ISSN:
0936-6768
,
1439-0531
DOI:
10.1111/rda.2018.53.issue-5
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2020494-2
SSG:
12
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