In:
Mediators of Inflammation, Hindawi Limited, Vol. 2012 ( 2012), p. 1-7
Abstract:
Objectives. In the present study, the ability of magnolol, a hydroxylated biphenyl compound isolated from Magnolia officinalis , to stimulate osteoblast function and inhibit the release of bone-resorbing mediators was investigated in osteoblastic MC3T3-E1 cells. Methods. Osteoblast function was measured by cell growth, alkaline phosphatase activity, collagen synthesis, and mineralization. Glutathione content was also measured in the cells. Bone-resorbing cytokines, receptor activator of nuclear factor- κ B ligand (RANKL), TNF- α , and IL-6 were measured with an enzyme immunoassay system. Results. Magnolol caused a significant elevation of cell growth, alkaline phosphatase activity, collagen synthesis, mineralization, and glutathione content in the cells ( P 〈 0.05 ). Skeletal turnover is orchestrated by a complex network of regulatory factors. Among cytokines, RANKL, TNF- α , and IL-6 were found to be key osteoclastogenetic molecules produced by osteoblasts. Magnolol significantly ( P 〈 0.05 ) decreased the production of osteoclast differentiation inducing factors such as RANKL, TNF- α , and IL-6 in the presence of antimycin A, which inhibits mitochondrial electron transport and has been used as an ROS generator. Conclusion. Magnolol might be a candidate as an agent for the prevention of bone disorders such as osteoporosis.
Type of Medium:
Online Resource
ISSN:
0962-9351
,
1466-1861
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2012
detail.hit.zdb_id:
2008065-7
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