In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_4 ( 2014-04-01), p. 1351-1358
Abstract:
Two facultatively anaerobic mesophilic bacteria, strains MEBiC 07026 T and MEBiC 08903 T , were isolated from two different tidal flat sediments and both strains showed approximately 92.2 % 16S rRNA gene sequence similarity with [ Cytophaga ] fermentans DSM 9555 T . 16S rRNA gene sequence similarity between the two new isolates was 97.5 % but levels of DNA–DNA relatedness between the two were 31.3–31.8 %. Phylogenetic analysis revealed that the two isolates and [ Cytophaga ] fermentans DSM 9555 T were affiliated with the family Marinilabiliaceae in the class Bacteroidia . The dominant fatty acids of strains MEBiC 07026 T , MEBiC 08903 T and [ Cytophaga ] fermentans DSM 9555 T were branched-type or hydroxylated C 15 : 0 , but [ Cytophaga ] fermentans DSM 9555 T contained a higher proportion of anteiso-branched fatty acids. The two new isolates contained a markedly higher proportion of monounsaturated fatty acids than other members of the family Marinilabiliaceae . The major respiratory quinone of the strains was MK-7. Strains MEBiC07026 T and MEBiC08903 T utilized a wide range of carboxylic acids whereas [ Cytophaga ] fermentans DSM 9555 T utilized carbohydrates rather than carboxylic acids. The DNA G+C content of the novel strains was about 44 mol% but that of [ Cytophaga ] fermentans DSM 9555 T revealed from the genome sequence was 37.6 mol%. Based on evidence from this polyphasic taxonomic study, a novel genus, Carboxylicivirga gen. nov., is proposed in the family Marinilabiliaceae with two novel species, Carboxylicivirga mesophila sp. nov. with type strain MEBiC 07026 T ( = KCCM 42978 T = JCM 18290 T ) and Carboxylicivirga taeanensis sp. nov. with type strain MEBiC 08903 T ( = KCCM 43024 T = JCM 19490 T ). Additionally, [ Cytophaga ] fermentans DSM 9555 T ( = ATCC 19072 T ) is reclassified as Saccharicrinis fermentans gen. nov., comb. nov.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.053462-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2014
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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