In:
Experimental Dermatology, Wiley, Vol. 24, No. 11 ( 2015-11), p. 857-863
Abstract:
Atopic dermatitis ( AD ) is a chronic inflammatory skin disease in which T‐helper type 2 (Th2)‐type immune responses are dominant. Th2 cytokine, interleukin ( IL )‐33 and thymic stromal lymphopoietin ( TSLP ) have been suggested to have an important role in AD . IL ‐33 is highly expressed in AD , but its role in AD has not yet been fully understood. To further identify the role of IL ‐33 in AD , we investigated the expression of TSLP induced by IL ‐33 in keratinocytes. This study revealed that IL ‐33 induced TSLP expression in human keratinocytes. Early growth response protein 1 (Egr)‐1, which is an inflammatory transcriptional factor, is induced by IL ‐33. IL ‐33‐mediated TSLP induction in keratinocytes was suppressed by treatment with mitogen‐activated protein kinase ( MAPK ) inhibitors or small interfering RNA against Egr‐1. Chromatin immunoprecipitation (Ch IP ) assay indicated the direct involvement of Egr‐1 in IL ‐33‐mediated TSLP induction. Taken together, these findings indicate that IL ‐33 may increase TSLP expression through an Egr‐1‐dependent mechanism via ERK 1/2, JNK and p38 activation in keratinocytes. These data suggest that the IL ‐33‐ ERK / JNK /p38/Egr‐1/ TSLP axis is involved in allergic skin Th2 inflammation, and it may be a novel therapeutic target.
Type of Medium:
Online Resource
ISSN:
0906-6705
,
1600-0625
DOI:
10.1111/exd.2015.24.issue-11
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2026228-0
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