In:
FEBS Letters, Wiley, Vol. 413, No. 2 ( 1997-08-18), p. 194-196
Abstract:
The addition of a poly‐His C‐terminal extension, designed to facilitate the purification of the protein, to the β‐lactamase of a thermophilic Bacillus licheniformis strain modified the site of action of the signal peptidase. This resulted in the secretion of a protein with a different N‐terminus, showing that this type of protein engineering might not always be as `neutral' as generally assumed.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(97)00908-3
Language:
English
Publisher:
Wiley
Publication Date:
1997
detail.hit.zdb_id:
212746-5
detail.hit.zdb_id:
1460391-3
SSG:
12
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