In:
Journal of Medical Microbiology, Microbiology Society, Vol. 56, No. 11 ( 2007-11-01), p. 1467-1473
Abstract:
A multiplex PCR assay has been developed for the identification of the six common Campylobacter taxa associated with human gastroenteritis and/or septicaemia, namely Campylobacter coli , Campylobacter fetus , Campylobacter hyointestinalis subsp. hyointestinalis , Campylobacter jejuni , Campylobacter lari and Campylobacter upsaliensis . The assay was developed using a combination of newly designed and published primers. It provided a specific PCR product for each of the five Campylobacter species and the one subspecies, and each of the PCR products was sufficiently distinguished by a difference in size by agarose gel electrophoresis. On evaluation of efficacy with 142 Campylobacter strains, the assay correctly identified all strains as 1 of the 6 Campylobacter taxa. This multiplex PCR assay is a rapid, simple and practical tool for identification of the six Campylobacter taxa commonly associated with gastroenteritis and/or septicaemia in humans, and offers an effective alternative to conventional biochemical-based assays.
Type of Medium:
Online Resource
ISSN:
0022-2615
,
1473-5644
DOI:
10.1099/jmm.0.47363-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2007
detail.hit.zdb_id:
2083944-3
SSG:
12
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