In:
Journal of General Virology, Microbiology Society, Vol. 94, No. 1 ( 2013-01-01), p. 90-96
Abstract:
Amino acid position 123 in the E protein of Japanese encephalitis virus (JEV) determines viral growth properties and pathogenicity. The majority of JEV strains have a serine residue at this position (E 123S ); however, JEV with an asparagine residue (E 123N ) has also been isolated. To compare the growth properties and pathogenicity of E 123S and E 123N JEV, we produced recombinant JEV with a serine-to-asparagine substitution at position 123 (rJEV-Mie41-E S123N ) in the E 123S -type strain Mie/41/2002 background. The growth rate of rJEV-Mie41-E S123N was similar to that of Mie/41/2002 in mammalian and mosquito cell lines. Mouse challenge experiments showed that there was only a slight difference in neuroinvasiveness between the parent strain (Mie/41/2002) and rJEV-Mie41-E S123N . Thus, our results indicate that the Ser-to-Asn substitution in the JEV E protein has weak impact on viral growth properties in vitro or on pathogenicity in vivo .
Type of Medium:
Online Resource
ISSN:
0022-1317
,
1465-2099
DOI:
10.1099/vir.0.044925-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2013
detail.hit.zdb_id:
2007065-2
SSG:
12
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