In:
Future Microbiology, Future Medicine Ltd, Vol. 15, No. 15 ( 2020-10), p. 1483-1487
Abstract:
RT-PCR-based assays for the detection of SARS-CoV-2 have played an essential role in the current COVID-19 pandemic. However, the sample collection and test reagents are in short supply, primarily due to supply chain issues. Thus, to eliminate testing constraints, we have optimized three key process variables: RNA extraction and RT-PCR reactions, different sample types and media to facilitate SARS-CoV-2 testing. By performing various validation and bridging studies, we have shown that various sample types such as nasopharyngeal swab, bronchioalveolar lavage and saliva, collected using conventional nasopharyngeal swabs, ESwab or 3D-printed swabs and, preserved in viral transport media, universal transport media, 0.9% sodium chloride or Amies media are compatible with RT-PCR assay for COVID-19. Besides, the reduction of PCR reagents by up to fourfold also produces reliable results.
Type of Medium:
Online Resource
ISSN:
1746-0913
,
1746-0921
DOI:
10.2217/fmb-2020-0094
Language:
English
Publisher:
Future Medicine Ltd
Publication Date:
2020
SSG:
12
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