In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 72, No. 8_Supplement ( 2012-04-15), p. 3198-3198
Abstract:
Molecular assays for the identification of rare allele occurrences are important tools for proper cancer classification and treatment. A prime example is the T790M mutation in EGFR which leads to resistance to the tyrosine kinase inhibitors gefitinib (Iressa®) and erlotinib (Tarceva®) used in the treatment of non-small cell lung cancer (NSCLC). Identification of the T790 mutation in cancer-shed particles in blood (either as whole cells or subcellular vesicles) calls out the need for an alternative cancer treatment. We have developed a highly sensitive PCR-based assay which allows the identification of the T790M mutation in blood plasma (either when present in mRNA or genomic DNA). The assay combines Real-Time PCR as well as melt curve analysis of the mutant PCR product and is followed by sequencing to verify the presence of the mutation. The Selector Assay is based on a wild-type specific PCR blocker and allows the mutant template to be amplified in a high background of wild-type template. A few copies of T790M mutant can be detected in greater than a 1000-fold excess of wild-type. Data using the Selector Assay with clinical lung cancer samples showing the identification of T790M in genomic DNA as well as mRNA in blood plasma and in CTCs will be presented. The Selector Assay can be applied to other mutations relevant to cancer and is a valuable tool for clinical diagnostics. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3198. doi:1538-7445.AM2012-3198
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2012-3198
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2012
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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