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  • 1
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    Online Resource
    Dysregulacja poziomu metylotransferaz argininy w patogenezie chorób nowotworowych
    Walter de Gruyter GmbH ; 2021
    In:  Postępy Higieny i Medycyny Doświadczalnej Vol. 75, No. 1 ( 2021-01-01), p. 272-282
    Details
    In: Postępy Higieny i Medycyny Doświadczalnej, Walter de Gruyter GmbH, Vol. 75, No. 1 ( 2021-01-01), p. 272-282
    Abstract: Metylacja argininy uznawana jest za jedną z najtrwalszych i najczęściej występujących modyfikacji potranslacyjnych. Reakcja przeniesienia grupy metylowej z S-adenyzolometioniny na aminową resztę argininy katalizowana jest przez metylotransferazy argininy (PRMT). W organizmie człowieka znanych jest dziewięć enzymów z rodziny PRMT, nazwanych zgodnie z kolejnością odkrycia PRMT1-PRMT9. Ze względu na produkt katalizowanej reakcji metylotransferazy argininy podzielono na trzy klasy: I, II, III. Produktami ich aktywności są odpowiednio: asymetryczna dimetyloarginina (ADMA), symetryczna dimetyloarginina (SDMA) oraz monometyloarginina (MMA). Powstałe modyfikacje w istotny sposób wpływają na strukturę chromatyny, dzięki czemu mogą pełnić funkcję koaktywatorów i supresorów transkrypcji. Metylacja argininy pełni wiele krytycznych funkcji, niezbędnych do prawidłowego funkcjonowania organizmu. Uczestniczy m.in. w kontroli transdukcji sygnału, splicingu mRNA oraz reguluje podstawowe procesy komórkowe, takie jak: proliferacja, różnicowanie, migracja i apoptoza. Coraz więcej dowodów wskazuje, że dysregulacja poziomu PRMT może powadzić do transformacji nowotworowej. Związek między podwyższonym poziomem PRMT a chorobą nowotworową udowodniono m.in. w raku: piersi, jajnika, płuc i jelita grubego. Aktywność metylotransferaz argininy może być regulowana za pomocą małocząsteczkowych inhibitorów PRMT. Obecnie trzy substancje hamujące aktywność PRMT znajdują się w fazie badań klinicznych i wykazują działanie przeciwnowotworowe wobec nowotworów hematologicznych. Przypuszcza się, że zastosowanie swoistych inhibitorów PRMT może się okazać nowym, skutecznym i bezpiecznym sposobem zwalczania chorób onkologicznych.
    Type of Medium: Online Resource
    ISSN: 1732-2693
    URL: Article
    DOI: 10.5604/01.3001.0014.8521
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2021
    detail.hit.zdb_id: 2150116-6
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  • 2
    Online Resource
    Online Resource
    Picropodophyllin (PPP) is a potent rhabdomyosarcoma growth inhibitor both in vitro and in vivo
    Springer Science and Business Media LLC ; 2017
    In:  BMC Cancer Vol. 17, No. 1 ( 2017-12)
    Details
    In: BMC Cancer, Springer Science and Business Media LLC, Vol. 17, No. 1 ( 2017-12)
    Type of Medium: Online Resource
    ISSN: 1471-2407
    URL: Article
    DOI: 10.1186/s12885-017-3495-y
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2017
    detail.hit.zdb_id: 2041352-X
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  • 3
    Online Resource
    Online Resource
    Expression of SCD and FADS2 Is Lower in the Necrotic Core and Growing Tumor Area than in the Peritumoral Area of Glioblastoma Multiforme
    MDPI AG ; 2020
    In:  Biomolecules Vol. 10, No. 5 ( 2020-05-07), p. 727-
    Details
    In: Biomolecules, MDPI AG, Vol. 10, No. 5 ( 2020-05-07), p. 727-
    Abstract: The expression of desaturases is higher in many types of cancer, and despite their recognized role in oncogenesis, there has been no research on the expression of desaturases in glioblastoma multiforme (GBM). Tumor tissue samples were collected during surgery from 28 patients (16 men and 12 women) diagnosed with GBM. The effect of necrotic conditions and nutritional deficiency (mimicking conditions in the studied tumor zones) was studied in an in vitro culture of human brain (glioblastoma astrocytoma) U-87 MG cells. Analysis of desaturase expression was made by qRT-PCR and the immunohistochemistry method. In the tumor, the expression of stearoyl–coenzyme A desaturase (SCD) and fatty acid desaturases 2 (FADS2) was lower than in the peritumoral area. The expression of other desaturases did not differ in between the distinguished zones. We found no differences in the expression of SCD, fatty acid desaturases 1 (FADS1), or FADS2 between the sexes. Necrotic conditions and nutritional deficiency increased the expression of the studied desaturase in human brain (glioblastoma astrocytoma) U-87 MG cells. The obtained results suggest that (i) biosynthesis of monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) in a GBM tumor is less intense than in the peritumoral area; (ii) expressions of SCD, SCD5, FADS1, and FADS2 correlate with each other in the necrotic core, growing tumor area, and peritumoral area; (iii) expressions of desaturases in a GBM tumor do not differ between the sexes; and (iv) nutritional deficiency increases the biosynthesis of MUFA and PUFA in GBM cells.
    Type of Medium: Online Resource
    ISSN: 2218-273X
    URL: Article
    DOI: 10.3390/biom10050727
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2701262-1
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  • 4
    Online Resource
    Online Resource
    The role of microRNAs in angiogenesis
    Pomorski Uniwersytet Medyczny w Szczecinie ; 2019
    In:  Pomeranian Journal of Life Sciences Vol. 64, No. 4 ( 2019-12-01), p. 37-41
    Details
    In: Pomeranian Journal of Life Sciences, Pomorski Uniwersytet Medyczny w Szczecinie, Vol. 64, No. 4 ( 2019-12-01), p. 37-41
    Abstract: Angiogenesis is the process that leads to the formation of new blood vessels. Under physiological conditions it occurs, inter alia, during corpus luteum formation and in some stages of the menstrual cycle. However, angiogenesis plays an essential role in many pathological conditions, particularly cancer. New blood vessel formation provides cancer cells with oxygen and essential nutrients, which stimulates tumor growth and facilitates its metastasis. Increasing evidence indicates that angiogenesis is regulated by microRNAs (miRNAs), which are small non-coding RNA molecules of 19–25 nucleotides. The main function of miRNAs is post-transcriptional regulation of gene expression, which controls many key biological processes, including cell proliferation, differentiation and migration. Endothelial miRNAs, known as angiomiRs, are presumably involved in tumor development and angiogenesis through regulation of pro- and antiangiogenic factors. To date, the miRNAs that stimulate angiogenesis are: miR-9, miR-27a, miR-30d, miR0-130b, miR-139, miR-146a, miR-150, miR-155, miR-200c, miR-296 and miR-558. Conversely, miRNAs that inhibit angiogenesis are: miR-145, miR-519c, miR-22, miR-20a, miR-92, miR-7b, miR-221, miR-222, miR-328 and miR-101.
    Type of Medium: Online Resource
    ISSN: 2719-6313
    URL: Article
    DOI: 10.21164/pomjlifesci.631
    Language: English
    Publisher: Pomorski Uniwersytet Medyczny w Szczecinie
    Publication Date: 2019
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  • 5
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    Online Resource
    Hymenolepis diminuta Infection Affects Apoptosis in the Small and Large Intestine
    MDPI AG ; 2022
    In:  International Journal of Environmental Research and Public Health Vol. 19, No. 15 ( 2022-08-08), p. 9753-
    Details
    In: International Journal of Environmental Research and Public Health, MDPI AG, Vol. 19, No. 15 ( 2022-08-08), p. 9753-
    Abstract: The rat tapeworm Hymenolepis diminuta has been shown to cause alterations in gastrointestinal tissues. Since hymenolepiasis induces a number of reactions in the host, it is reasonable to assume that it may also be involved in the mechanisms of apoptosis in the intestines. Individual research tasks included an examination of the effect of H. diminuta infection on; (i) the cellular localization of the expression of pro-apoptotic protein Bax and anti-apoptotic protein Bcl-2, as well as caspase-3 and caspase-9, and (ii) the effects of the infection on the expression of Bcl-2, Bax, Cas-3 and Cas-9, at the mRNA and protein levels. Molecular tests (including mRNA (qRT PCR) and the protein (Western blot) expression of Bax, Bcl-2, and caspases-3, -9) and immunohistochemical tests were performed during the experiment. They showed that H. diminuta infection activates the intrinsic apoptosis pathway in the small and large intestine of the host. H. diminuta infection triggered the apoptosis via the activation of the caspase cascade, including Cas-3 and Cas-9. Hymenolepiasis enhanced apoptosis in the small and large intestine of the host by increasing the expression of the pro-apoptotic gene and protein Bax and by decreasing the expression of the anti-apoptotic gene and protein Bcl-2.
    Type of Medium: Online Resource
    ISSN: 1660-4601
    URL: Article
    DOI: 10.3390/ijerph19159753
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2175195-X
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  • 6
    Online Resource
    Online Resource
    CCL2 Gene Expression and Protein Level Changes Observed in Response to Wingate Anaerobic Test in High-Trained Athletes and Non-Trained Controls
    MDPI AG ; 2022
    In:  International Journal of Environmental Research and Public Health Vol. 19, No. 16 ( 2022-08-12), p. 9947-
    Details
    In: International Journal of Environmental Research and Public Health, MDPI AG, Vol. 19, No. 16 ( 2022-08-12), p. 9947-
    Abstract: Intensive, acute exercise may bring a large systemic inflammatory response marked by substantial increases in inflammatory cytokines and chemokines. One such chemokines–CCL2–is a key factor involved in inflammatory reaction to exercise. The direct aim of the study was to describe the changes in the CCL2 expression levels after anaerobic exercise in well-trained athletes adapted to long-term training and in non-trained participants. The expression of CCL2 mRNA was evaluated in peripheral blood MNCs and CCL2 protein level was observed in blood plasma. The changes were assessed as the response to an acute, intensive bout of exercise (Wingate Anaerobic Test) in two groups of participants: well-trained soccer players and non-trained individuals. An increase of CCL2 expression inn both mRNA and protein levels was observed. The response was greater in non-trained individuals and elevated levels of CCL2 transcripts persisted for more than 24 h after exercise. Well-trained individuals responded more modestly and the effect was attenuated relatively quickly. This shows muscular adaptation to a continuous training regime in well-trained individuals and better control of immune reactions to muscular injury. In non-training individuals, the induction of the inflammatory response was greater, suggesting presence of more serious myotrauma.
    Type of Medium: Online Resource
    ISSN: 1660-4601
    URL: Article
    DOI: 10.3390/ijerph19169947
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2175195-X
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  • 7
    Online Resource
    Online Resource
    Garcinol—A Natural Histone Acetyltransferase Inhibitor and New Anti-Cancer Epigenetic Drug
    MDPI AG ; 2021
    In:  International Journal of Molecular Sciences Vol. 22, No. 6 ( 2021-03-11), p. 2828-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 22, No. 6 ( 2021-03-11), p. 2828-
    Abstract: Garcinol extracted from Garcinia indica fruit peel and leaves is a polyisoprenylated benzophenone. In traditional medicine it was used for its antioxidant and anti-inflammatory properties. Several studies have shown anti-cancer properties of garcinol in cancer cell lines and experimental animal models. Garcinol action in cancer cells is based on its antioxidant and anti-inflammatory properties, but also on its potency to inhibit histone acetyltransferases (HATs). Recent studies indicate that garcinol may also deregulate expression of miRNAs involved in tumour development and progression. This paper focuses on the latest research concerning garcinol as a HAT inhibitor and miRNA deregulator in the development and progression of various cancers. Garcinol may be considered as a candidate for next generation epigenetic drugs, but further studies are needed to establish the precise toxicity, dosages, routes of administration, and safety for patients.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms22062828
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 8
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    Protein Arginine Methyltransferase (PRMT) Inhibitors—AMI-1 and SAH Are Effective in Attenuating Rhabdomyosarcoma Growth and Proliferation in Cell Cultures
    MDPI AG ; 2021
    In:  International Journal of Molecular Sciences Vol. 22, No. 15 ( 2021-07-27), p. 8023-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 22, No. 15 ( 2021-07-27), p. 8023-
    Abstract: Rhabdomyosarcoma (RMS) is a malignant soft tissue cancer that develops mostly in children and young adults. With regard to histopathology, four rhabdomyosarcoma types are distinguishable: embryonal, alveolar, pleomorphic and spindle/sclerosing. Currently, increased amounts of evidence indicate that not only gene mutations, but also epigenetic modifications may be involved in the development of RMS. Epigenomic changes regulate the chromatin architecture and affect the interaction between DNA strands, histones and chromatin binding proteins, thus, are able to control gene expression. The main aim of the study was to assess the role of protein arginine methyltransferases (PRMT) in the cellular biology of rhabdomyosarcoma. In the study we used two pan-inhibitors of PRMT, called AMI-1 and SAH, and evaluated their effects on proliferation and apoptosis of RMS cells. We observed that AMI-1 and SAH reduce the invasive phenotype of rhabdomyosarcoma cells by decreasing their proliferation rate, cell viability and ability to form cell colonies. In addition, microarray analysis revealed that these inhibitors attenuate the activity of the PI3K-Akt signaling pathway and affect expression of genes related to it.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms22158023
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 9
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    Epigenetic Regulation of Inflammatory Responses in the Context of Physical Activity
    MDPI AG ; 2021
    In:  Genes Vol. 12, No. 9 ( 2021-08-25), p. 1313-
    Details
    In: Genes, MDPI AG, Vol. 12, No. 9 ( 2021-08-25), p. 1313-
    Abstract: Epigenetic modifications occur in response to environmental changes and play a fundamental role in the regulation of gene expression. PA is found to elicit an inflammatory response, both from the innate and adaptive divisions of the immunological system. The inflammatory reaction is considered a vital trigger of epigenetic changes that in turn modulate inflammatory actions. The tissue responses to PA involve local and general changes. The epigenetic mechanisms involved include: DNA methylation, histone proteins modification and microRNA. All of them affect genetic expression in an inflammatory milieu in physical exercise depending on the magnitude of physiological stress experienced by the exerciser. PA may evoke acute or chronic biochemical and physiological responses and have a positive or negative immunomodulatory effect.
    Type of Medium: Online Resource
    ISSN: 2073-4425
    URL: Article
    DOI: 10.3390/genes12091313
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2527218-4
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  • 10
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    Rola microRNA w różnicowaniu mięśni szkieletowych
    Pomorski Uniwersytet Medyczny w Szczecinie ; 2018
    In:  Pomeranian Journal of Life Sciences Vol. 63, No. 4 ( 2018-01-09)
    Details
    In: Pomeranian Journal of Life Sciences, Pomorski Uniwersytet Medyczny w Szczecinie, Vol. 63, No. 4 ( 2018-01-09)
    Abstract: Miogeneza jest skomplikowanym i wieloetapowym procesem rozpoczynającym się w życiu płodowym. Rozwój mięśni szkieletowych regulowany jest przez liczne czynniki oraz ścieżki sygnalizacyjne, które mogą być kontrolowane przez microRNA. MicroRNA to krótkie, niekodujące odcinki RNA pochodzenia endogennego, o długości 21–25 nukleotydów. MicroRNA odgrywają istotną rolę w mechanizmach potranskrypcyjnej regulacji ekspresji genów poprzez wiązanie z komplementarnym mRNA, powodując jego degradację lub inhibicję translacji białka. Wykryto, iż niektóre miRNA występują jedynie w tkance mięśniowej, odgrywając szczególnie ważną rolę w procesie różnicowania komórek mięśniowych. Do grupy mięśniowo specyficznych miRNA (myomiRs) zalicza się: miR-1, miR-27b, miR-133, miR-195, miR-199, miR-206, miR-499. Stan podniesionego stężenia mięśniowo charakterystycznych microRNA jest typowy dla późnego stadium różnicowania komórek mięśniowych, a ich ilość jest wprost proporcjonalna do zdolności różnicowania się mioblastów w miotubule. Dysregulacja stężenia myomiRs może prowadzić do rozwoju chorób mięśni szkieletowych, w tym: mięsaka prążkowanokomórkowego, dystrofii mięśniowej Duchenne’a, stwardnienia zanikowego bocznego.
    Type of Medium: Online Resource
    ISSN: 2450-4637
    URL: Article
    DOI: 10.21164/pomjlifesci.345
    Language: Unknown
    Publisher: Pomorski Uniwersytet Medyczny w Szczecinie
    Publication Date: 2018
    detail.hit.zdb_id: 2856105-3
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