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  • 1
    Online Resource
    Online Resource
    Expression of CD200/CD200R regulatory molecules on granulocytes and monocytes is modulated by cardiac surgical operation
    SAGE Publications ; 2010
    In:  Perfusion Vol. 25, No. 6 ( 2010-11), p. 389-397
    Details
    In: Perfusion, SAGE Publications, Vol. 25, No. 6 ( 2010-11), p. 389-397
    Abstract: Aims: Cardiac surgical operation is inseparably linked to the induction of an inflammatory response. Both humoral and cellular regulatory mechanisms are operating to maintain body homeostasis. We followed the changes in the expression of CD200/CD200R regulatory molecules on monocytes and granulocyte of cardiac surgical patients operated on using either standard (OP) or modified “mini-invasive” cardiopulmonary bypass (MOP). Methods: Expression of CD200/CD200R regulatory molecules was determined by flow cytometry. Results: The expression of CD200R on granulocytes was increased after surgery in both groups of patients, but the increase was statistically significant only in OP patients (p 〈 0.01). At this time point, there was a significant difference in CD200R expression on granulocytes when comparing OP to MOP patients, being higher in the former group (p 〈 0.01). The expression of CD200R on monocytes was diminished after surgery and during an early postoperative period in both groups of patients. The expression of CD200 on monocytes was significantly diminished after surgery in both groups (p 〈 0.01). Nonetheless, we observed an increase in CD200 expression in OP patients at the 3 rd postoperative day. There was a statistically significantly increased CD200 expression on monocytes of OP patients (p 〈 0.001) at the 3 rd postoperative day when we compared OP and MOP groups. The expression of CD200 on granulocytes was significantly higher after surgery and at the 3 rd postoperative day in OP when compared to MOP patients. Conclusions: CD200R expression on granulocytes was significantly increased, while CD200 and CD200R expression on monocytes was decreased after cardiac surgery.
    Type of Medium: Online Resource
    ISSN: 0267-6591 , 1477-111X
    URL: Article
    DOI: 10.1177/0267659110381451
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2010
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  • 2
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    Could Pentraxin 3 Be a New Diagnostic Marker for Excessive Inflammatory Response in Cardiac Surgery?
    Georg Thieme Verlag KG ; 2014
    In:  The Thoracic and Cardiovascular Surgeon Vol. 62, No. 08 ( 2014-8-22), p. 670-676
    Details
    In: The Thoracic and Cardiovascular Surgeon, Georg Thieme Verlag KG, Vol. 62, No. 08 ( 2014-8-22), p. 670-676
    Type of Medium: Online Resource
    ISSN: 0171-6425 , 1439-1902
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.1055/s-004-28224
    DOI: 10.1055/s-00000085
    DOI: 10.1055/s-0034-1384668
    Language: English
    Publisher: Georg Thieme Verlag KG
    Publication Date: 2014
    detail.hit.zdb_id: 2056554-9
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  • 3
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    Serum level of sCD163, a soluble receptor for hemoglobin, is influenced by cardiac surgery
    SAGE Publications ; 2009
    In:  Perfusion Vol. 24, No. 4 ( 2009-07), p. 263-269
    Details
    In: Perfusion, SAGE Publications, Vol. 24, No. 4 ( 2009-07), p. 263-269
    Abstract: Background:The scavenger receptor for complexes hemoglobin-haptoglobin (CD163), which is expressed on monocytes/ macrophages, is shed to the body fluids in a soluble form (sCD163). Objectives: To evaluate the dynamics of sCD163 in the blood of patients undergoing cardiac surgery. Patients and Methods: Sixty-one adult patients who underwent coronary artery bypass grafting (CABG) were enrolled in the study. They were assigned to undergo CABG using either cardiopulmonary bypass (CPB), “on-pump”, (22 patients), modified CPB, mini “on-pump”, (17 patients) or without CPB, “off-pump”, (22 patients) surgery. Serum levels of sCD163 in venous blood samples taken before and after surgery, and during an early postoperative period, were evaluated by Macro 163 TM diagnostic kit (IQ Products, Groningen, NL). Results: Compared to the preoperative levels (“on-pump”; 344 ng/mL, “off-pump”; 314.5 ng/mL, mini-invasive “on-pump”; 336.5 ng/mL) serum levels were elevated at the finish of surgery, reaching maximum at the 1 st postoperative day (“onpump”; 658 ng/mL; p 〈 0.05, “off-pump”; 810.5 ng/mL; p 〈 0.01; mini-invasive “on-pump”; 663 ng/mL; non-significant). No significant differences regarding the serum levels of sCD163 between different surgical approaches were found. Conclusion: Serum level of sCD163 scavenger molecule for hemoglobin is elevated at the end of surgery and at the 1 st postoperative day, being little influenced by cardiopulmonary bypass.
    Type of Medium: Online Resource
    ISSN: 0267-6591 , 1477-111X
    URL: Article
    DOI: 10.1177/0267659109348723
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2009
    detail.hit.zdb_id: 2029611-3
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  • 4
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    Expression of urokinase plasminogen activator receptor on monocytes and granulocytes is modulated by cardiac surgery
    SAGE Publications ; 2011
    In:  Perfusion Vol. 26, No. 2 ( 2011-03), p. 115-121
    Details
    In: Perfusion, SAGE Publications, Vol. 26, No. 2 ( 2011-03), p. 115-121
    Abstract: Aims: Coronary artery bypass grafting (CABG) is linked to the induction of the blood coagulation/fibrinolysis cascade, which is an integral component of inflammation induced by cardiac surgery. We followed the modulation of urokinase plasminogen activator receptor uPAR (CD87) separately for monocytes and granulocytes in blood of cardiac surgery patients. Methods: Expression of uPAR, analyzed as Median Fluorescence Intensity (MFI), on blood monocytes and granulocytes was determined by flow cytometry. Changes in uPAR expression in patients undergoing CABG using standard cardiopulmonary bypass (“on-pump”) were compared to the changes in uPAR expression in patients undergoing CABG using mini-invasive cardiopulmonary bypass (“mini on-pump”). Results: In “on-pump” patients, the median of uPAR expression on granulocytes before surgery was 18.1 (InterQuartile Range (IQR): 15.6-20.4). uPAR expression was significantly decreased after surgery (p 〈 0.001), on the first postoperative day (p 〈 0.001), and on the third postoperative day (p 〈 0.05). In “mini on-pump” patients, the median of uPAR expression on granulocytes before surgery was 15.2 (IRQ: 13.8-19.4). The significantly decreased uPAR expression was found only at the end of surgery (p 〈 0.05). The similar pattern of uPAR expression was also found for monocytes. The preoperative level in “on-pump” patients was 23.3 (IRQ: 18.9-30.2). There was significantly decreased uPAR expression at the end of surgery (p 〈 0.01) and at the first postoperative day (p 〈 0.05). In “mini on-pump” patients, the preoperative uPAR expression was 16.9 (IQR: 14.5-20.2). Expression of uPAR was significantly decreased only after surgery (p 〈 0.05). When comparing “onpump” patients to “mini on-pump” patients, no significant differences in the expression of uPAR were found. Conclusion: uPAR expression on granulocytes and monocytes is significantly modulated by cardiac surgery.
    Type of Medium: Online Resource
    ISSN: 0267-6591 , 1477-111X
    URL: Article
    DOI: 10.1177/0267659110389845
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2011
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  • 5
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    Effects of conventional CPB and mini-CPB on neutrophils CD162, CD166 and CD195 expression
    SAGE Publications ; 2017
    In:  Perfusion Vol. 32, No. 2 ( 2017-03), p. 141-150
    Details
    In: Perfusion, SAGE Publications, Vol. 32, No. 2 ( 2017-03), p. 141-150
    Abstract: Cardiac surgery is known to trigger a systemic inflammatory response. While the use of conventional cardiopulmonary bypass (CPB) results in profound inflammation, modified mini-CPB is considered less harmful. We evaluated the impact of cardiac surgery on the expression of CD162, CD166, CD195 molecules and their association with the type of CPB used. Methods and Results: Twenty-four patients were enrolled in our study. Twelve of them were operated using conventional CPB while the other twelve patients underwent surgery with mini-CPB. Blood samples were analysed by flow cytometry. We observed a significant increase in median fluorescence intensity of CD162 and CD195 that peaked instantly after surgery and normalized to the baseline value on the 1 st day post surgery, whereas CD166 was initially down-regulated and its median fluorescence intensity (MFI) value increased to the baseline in the next few days. Conclusion: We observed immediate changes in the expression of CD162, CD166, and CD195 molecules on the neutrophils after surgery in both study groups of patients. The intensity of the observed changes was significantly greater in the group of patients who underwent conventional CPB compared to patients who underwent mini-CPB cardiac surgery.
    Type of Medium: Online Resource
    ISSN: 0267-6591 , 1477-111X
    URL: Article
    DOI: 10.1177/0267659116669586
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2017
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  • 6
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    The Effect of Conventional and Mini-Invasive Cardiopulmonary Bypass on Neutrophil Activation in Patients Undergoing Coronary Artery Bypass Grafting
    Hindawi Limited ; 2012
    In:  Mediators of Inflammation Vol. 2012 ( 2012), p. 1-8
    Details
    In: Mediators of Inflammation, Hindawi Limited, Vol. 2012 ( 2012), p. 1-8
    Abstract: Interleukin-10 (IL-10) is considered to be a cytokine with potent anti-inflammatory properties, which have been previously linked to increased incidence of sepsis. The level of IL-10 is elevated by cardiac surgery when cardiopulmonary bypass (CPB) and methylprednisolone are used. In our study, we compare the level of IL-10, IL-10 Receptor (IL-10R), and percentage of neutrophils between two groups of cardiac surgical patients undergoing Coronary Artery Bypass Grafting, both of which were not given methylprednisolone. The first group was operated with conventional CPB, while the second group was operated with minimally invasive CPB (mini-CPB). We detected enhanced level of IL-10 during surgery and at the end of surgery in both groups of patients. While no correlation between IL-10 and IL10R was found, IL-10 was positively correlated with increased percentage of neutrophils at the time points when the level of IL-10 peaked.
    Type of Medium: Online Resource
    ISSN: 0962-9351 , 1466-1861
    URL: Article
    DOI: 10.1155/2012/152895
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2012
    detail.hit.zdb_id: 2008065-7
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  • 7
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    Lipopolysaccharide Binding Protein and sCD14 are Not Produced as Acute Phase Proteins in Cardiac Surgery
    Hindawi Limited ; 2007
    In:  Mediators of Inflammation Vol. 2007 ( 2007), p. 1-6
    Details
    In: Mediators of Inflammation, Hindawi Limited, Vol. 2007 ( 2007), p. 1-6
    Abstract: Objectives . The changes in the serum levels of lipopolysaccharide binding protein (LBP) and sCD14 during cardiac surgery were followed in this study. Design . Thirty-four patients, 17 in each group, were randomly assigned to coronary artery bypass grafting surgery performed either with (“on-pump”) or without (“off-pump”) cardiopulmonary bypass. LBP and sCD14 were evaluated by ELISA. Results . The serum levels of LBP were gradually increased from the 1st postoperative day and reached their maximum on the 3rd postoperative day in both “on-pump” and “off-pump” patients ( 30.33 ± 9.96 μ g/mL; 37.99 ± 16.58 μ g/mL), respectively. There were no significant differences between “on-pump” and “off-pump” patients regarding LBP. The significantly increased levels of sCD14 from the 1st up to the 7th postoperative day in both “on-pump” and “off-pump” patients were found with no significant differences between these groups. No correlations between LBP and sCD14 and IL-6, CRP and long pentraxin PTX3 levels were found. Conclusions . The levels of LBP and sCD14 are elevated in cardiac surgical patients being similar in both groups. These molecules are not produced as acute phase proteins in these patients.
    Type of Medium: Online Resource
    ISSN: 0962-9351 , 1466-1861
    URL: Article
    DOI: 10.1155/2007/72356
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2007
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  • 8
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    The long pentraxin 3 in cardiac surgery: Distinct responses in “on-pump” and “off-pump” patients
    Informa UK Limited ; 2007
    In:  Scandinavian Cardiovascular Journal Vol. 41, No. 3 ( 2007-01), p. 171-179
    Details
    In: Scandinavian Cardiovascular Journal, Informa UK Limited, Vol. 41, No. 3 ( 2007-01), p. 171-179
    Type of Medium: Online Resource
    ISSN: 1401-7431 , 1651-2006
    URL: Article
    DOI: 10.1080/14017430701324262
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2007
    detail.hit.zdb_id: 1497073-9
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  • 9
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    Impact of cardiac surgery on the expression of CD40, CD80, CD86 and HLA-DR on B cells and monocytes
    SAGE Publications ; 2016
    In:  Perfusion Vol. 31, No. 5 ( 2016-07), p. 391-400
    Details
    In: Perfusion, SAGE Publications, Vol. 31, No. 5 ( 2016-07), p. 391-400
    Abstract: We measured and compared changes in the percentage of cells expressing CD80, CD86, CD40, HLA-DR and the expression of these molecules on B cells and monocytes of patients who underwent either on-pump, mini on-pump or off-pump cardiac surgery. Methods: Blood samples from patients who underwent either on-pump, mini on-pump or off-pump cardiac surgery were collected before surgery, instantly after surgery and on the 1 st , 3 rd and 7 th days after surgery. Surface expression of CD80, CD86, CD40 and HLA-DR molecules was determined by flow cytometry. Results: Our results show that all three surgical techniques altered the expression of these molecules, as well as the percentage relative number of specific cell populations. We identified statistically significant differences when comparing different surgical techniques. On-pump surgery revealed a more pronounced impact on the phenotype of immune system cells than the other techniques. Therefore, it is likely that the function of immune cells is changed the most by on-pump surgery. We found a lower decrease in the number of CD80 + monocytes and a lower drop in the CD40 expression on monocytes in off-pump patients in comparison with on-pump patients. Conclusion: All the types of cardiac surgical techniques, off-pump, on-pump and modified mini-invasive on-pump, are associated with changes in CD80, CD86, CD40 and HLA-DR expression. We found several significant differences in the expression of the selected molecules when we compared all three groups of patients.
    Type of Medium: Online Resource
    ISSN: 0267-6591 , 1477-111X
    URL: Article
    DOI: 10.1177/0267659115612905
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2016
    detail.hit.zdb_id: 2029611-3
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  • 10
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    Early Expression of Fc γ RI (CD64) on Monocytes of Cardiac Surgical Patients and Higher Density of Monocyte Anti-Inflammatory Scavenger CD163 Receptor in “On-Pump” Patients
    Hindawi Limited ; 2008
    In:  Mediators of Inflammation Vol. 2008 ( 2008), p. 1-6
    Details
    In: Mediators of Inflammation, Hindawi Limited, Vol. 2008 ( 2008), p. 1-6
    Abstract: Objective . Activation of innate immunity cells is inseparably linked to cardiac surgical operation. The aim of this study was to assess the kinetics in the expression of receptor for Fc part of IgG, Fc γ RI (CD64), and scavenger receptor CD163 on peripheral blood cells of cardiac surgical patients and to examine the effect of cardiac bypass as a separable influence on the systemic acute inflammatory response. Methods . Forty patients, twenty in each group, were randomly assigned to CABG surgery performed either with “on-pump” or without “off-pump” cardiopulmonary bypass. Standardized quantitative flow cytometry method was used to determine the expression of surface markers. Results . The density of CD64 molecule on monocytes reached maximum on the 1st postoperative day ( P 〈 .001 ) whereas the peak for CD64 molecule expression on granulocytes was postponed to the 3rd postoperative day ( P 〈 .001 ). The expression of CD163 scavenger molecule on monocytes reached maximum on the 1st postoperative day ( P 〈 .001 ). The density of CD163 molecule on monocytes on the 1st postoperative day is significantly higher in “on-pump” patients in comparison with “off-pump” patients ( P 〈 .001 ). Conclusion . In cardiac surgical patients the expression of activation marker Fc γ R1 (CD64) on monocytes is increased earlier in comparison with granulocytes in both “on-pump” and “off-pump” patients. The expression of scavenger molecule CD163 on monocytes is significantly higher in “on-pump” patients.
    Type of Medium: Online Resource
    ISSN: 0962-9351 , 1466-1861
    URL: Article
    DOI: 10.1155/2008/235461
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2008
    detail.hit.zdb_id: 2008065-7
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