In:
The FASEB Journal, Wiley, Vol. 24, No. S1 ( 2010-04)
Abstract:
Vascular complications in diabetes mellitus (DM) are associated with increased reactive oxygen species (ROS) formation. Therefore we tested, if treatment with PETN, which has been shown to induce the antioxidant enzyme heme oxygenase‐1, improves ROS formation and vascular function in a diabetic rat model. Methods/Results DM was induced by a single i.v. injection of streptozotocin (STZ, 60mg/kg) in male Wistar rats. After 8 weeks of PETN (15mg/kg/d) vs. isosorbid dinitrate (ISDN, 10 mg/kg/d) vs. isosorbide‐5‐mononitrate (ISMN, 75mg/kg/d) treatment, rats were sacrificed. STZ‐treated rats showed an increase in vascular and cardiac ROS production, detected by HPLC. Endothelial function, measured by isometric tension studies, was impaired. PETN therapy improved almost all parameters more efficiently than ISDN, while ISMN had no protective effects. Some rats were treated with insulin (2.5U/d). Insulin normalized all parameters completely proving that STZ‐induced vascular dysfunction depends on hyperglycemia but not on unspecific toxicity of the compound. Conclusion In diabetic rats, PETN showed more pronounced antioxidative effects than ISDN. Insulin normalized all parameters and thereby identified the underlying mechanism of cardiovascular dysfunction in STZ‐induced DM to strictly depend on insulin levels highlighting the importance of this model.
Type of Medium:
Online Resource
ISSN:
0892-6638
,
1530-6860
DOI:
10.1096/fasebj.24.1_supplement.571.9
Language:
English
Publisher:
Wiley
Publication Date:
2010
detail.hit.zdb_id:
1468876-1
detail.hit.zdb_id:
639186-2
SSG:
12
Permalink
