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  • Kiyoshima, Tamotsu  (3)
  • Sakai, Takako  (3)
  • 1
    Online Resource
    Online Resource
    Protogenin, a new member of the immunoglobulin superfamily, is implicated in the development of the mouse lower first molar
    Springer Science and Business Media LLC ; 2010
    In:  BMC Developmental Biology Vol. 10, No. 1 ( 2010-12)
    Details
    In: BMC Developmental Biology, Springer Science and Business Media LLC, Vol. 10, No. 1 ( 2010-12)
    Abstract: Protogenin (Prtg) has been identified as a gene which is highly expressed in the mouse mandible at embryonic day 10.5 (E10.5) by a cDNA subtraction method between mandibles at E10.5 and E12.0. Prtg is a new member of the deleted in colorectal carcinoma (DCC) family, which is composed of DCC, Neogenin, Punc and Nope. Although these members play an important role in the development of the embryonic central nervous system, recent research has also shed on the non-neuronal organization. However, very little is known regarding the fetal requirement of the non-neuronal organization for Prtg and how this may be associated with the tooth germ development. This study examined the functional implications of Prtg in the developing tooth germ of the mouse lower first molar. Results Ptrg is preferentially expressed in the early stage of organogenesis. Prtg mRNA and protein were widely expressed in the mesenchymal cells in the mandible at E10.5. The oral epithelial cells were also positive for Prtg. The expression intensity of Prtg after E12.0 was markedly reduced in the mesenchymal cells of the mandible, and was restricted to the area where the tooth bud was likely to be formed. Signals were also observed in the epithelial cells of the tooth germ. Weak signals were observed in the inner enamel epithelial cells at E16.0 and E18.0. An inhibition assay using a hemagglutinating virus of Japan-liposome containing Prtg antisense-phosphorothioated-oligodeoxynucleotide (AS-S-ODN) in cultured mandibles at E10.5 showed a significant growth inhibition in the tooth germ. The relationship between Prtg and the odontogenesis-related genes was examined in mouse E10.5 mandible, and we verified that the Bmp-4 expression had significantly been decreased in the mouse E10.5 mandible 24 hr after treatment with Prtg AS-S-ODN. Conclusion These results indicated that the Prtg might be related to the initial morphogenesis of the tooth germ leading to the differentiation of the inner enamel epithelial cells in the mouse lower first molar. A better understanding of the Prtg function might thus play a critical role in revealing a precious mechanism in tooth germ development.
    Type of Medium: Online Resource
    ISSN: 1471-213X
    URL: Article
    DOI: 10.1186/1471-213X-10-115
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2010
    detail.hit.zdb_id: 2041492-4
    SSG: 12
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  • 2
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    Online Resource
    Age‐Dependent Changes in the Distribution of BrdU‐ and TUNEL‐Positive Cells in the Murine Gingival Tissue
    Wiley ; 1999
    In:  Journal of Periodontology Vol. 70, No. 9 ( 1999-09), p. 973-981
    Details
    In: Journal of Periodontology, Wiley, Vol. 70, No. 9 ( 1999-09), p. 973-981
    Abstract: Background : Age‐dependent morphological and cell kinetic changes of the gingival tissue seem to be related to the occurrence of periodontal disease. The purpose of this study was to investigate the age‐dependent changes in the distribution of BrdU‐ and TUNEL‐positive cells in murine gingival tissue. Methods : Gingival tissue of the lower first molar region of 2‐, 3‐, 5‐, 7‐, 10‐, 15‐, 20‐, 30‐, 40‐, 50‐, 60‐, 70‐ and 80‐week‐old mice was used in this study. BrdU‐ and TUNEL‐positive cells were evaluated at the following 4 sites: 1) gingival epithelium (GE); 2) junctional epithelium (JE); 3) submucosal connective tissue of the gingival epithelium (GECT); and 4) submucosal connective tissue of the junctional epithelium (JECT). Results : No significant differences in the mean number of BrdU‐positive cells at each site were demonstrated among the various age groups. No significant change in the mean number of TUNEL‐positive cells was demonstrated in either the GE or JE groups among the various age groups. Meanwhile, a significant increase in the TUNEL‐positive cells was observed in the GECT of mice 40 weeks or older, and in the JECT of mice 20 weeks or older. Conclusions : These results indicate that no age‐dependent change in the cell proliferation or cell death occurred in the gingival and junctional epithelial layers as well as in the cell proliferation in the submucosal connective tissue. Meanwhile, a significant decrease in the cellular component of the submucosal connective tissue of both gingival and junctional epithelial layers caused by apoptosis occurred with aging. The decreased cellular component in the submucosal connective tissue thus seems to be related to either gingival recession or to the apical migration of the JE with aging. These morphological changes with aging possibly occur in humans and may be related to the susceptibility to periodontal disease in aged individuals. J Periodontol 1999;70:973‐981.
    Type of Medium: Online Resource
    ISSN: 0022-3492 , 1943-3670
    URL: Article
    DOI: 10.1902/jop.1999.70.9.973
    Language: English
    Publisher: Wiley
    Publication Date: 1999
    detail.hit.zdb_id: 2040047-0
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  • 3
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    Age-dependent changes in cell proliferation and cell death in the periodontal tissue and the submandibular gland in mice: a comparison with other tissues and organs
    Springer Science and Business Media LLC ; 2007
    In:  Journal of Molecular Histology Vol. 38, No. 4 ( 2007-8), p. 321-332
    Details
    In: Journal of Molecular Histology, Springer Science and Business Media LLC, Vol. 38, No. 4 ( 2007-8), p. 321-332
    Type of Medium: Online Resource
    ISSN: 1567-2379 , 1567-2387
    URL: Article
    DOI: 10.1007/s10735-007-9105-6
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2007
    detail.hit.zdb_id: 1483720-1
    SSG: 12
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