In:
Journal of Biomedicine and Biotechnology, Hindawi Limited, Vol. 2003, No. 5 ( 2003), p. 299-307
Abstract:
Protein microarray-based approaches are increasingly being used in research and clinical applications to either profile the expression of proteins or screen molecular interactions. The development of high-throughput, sensitive, convenient, and cost-effective formats for detecting proteins is a necessity for the effective advancement of understanding disease processes. In this paper, we describe the generation of highly multiplexed, antibody-based, specific, and sensitive protein microarrays coupled with rolling-circle signal amplification (RCA) technology. A total of 150 cytokines were simultaneously detected in an RCA sandwich immunoassay format. Greater than half of these proteins have detection sensitivities in the pg/ml range. The validation of antibody microarray with human serum indicated that RCA-based protein microarrays are a powerful tool for high-throughput analysis of protein expression and molecular diagnostics.
Type of Medium:
Online Resource
ISSN:
1110-7243
,
1110-7251
DOI:
10.1155/S1110724303209268
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2003
detail.hit.zdb_id:
2698540-8
detail.hit.zdb_id:
2512507-2
SSG:
12
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