In:
American Journal of Reproductive Immunology, Wiley, Vol. 62, No. 6 ( 2009-12), p. 352-356
Abstract:
Problem The intracellular antioxidant system, based on glutathione (GSH), plays a key role in endometrial detoxification reactions and has been proposed to be involved in the pathogenesis endometriosis. This study was designed to evaluate whether estradiol (E 2 ) and proinflammatory cytokines have any effects on expression of glutathione in endometrial stromal cells (ESCs). Method of study Glutathione levels were measured utilizing high‐performance liquid chromatography following in vitro culture and treatment of ESCs with estradiol, tumor necrosis factor‐alpha (TNF‐α) and interleukin 1‐beta (IL‐1β). Results The GSH level in E 2 (10 −8 m ) treatment group was significantly higher than in the control group at 48 h ( P 〈 0.05). In vitro treatment of ESCs with TNF‐α 10 ng/mL as well as E 2 (10 −8 m ) plus TNF‐α 10 ng/mL for 48 hr also led to a significant increase in GSH level ( P 〈 0.05; P 〈 0.05, respectively). Both IL‐1β 10 ng/mL and E 2 (10 −8 m ) plus IL‐1β 10 ng/mL for 48 hr increased GSH level significantly ( P 〈 0.05; P 〈 0.05, respectively) as well. Conclusions These findings might suggest that increased production of estradiol and proinflammatory cytokines in the peritoneal cavity possibly leads to the establishment of endometriosis through increased level of GSH.
Type of Medium:
Online Resource
ISSN:
1046-7408
,
1600-0897
DOI:
10.1111/aji.2009.62.issue-6
DOI:
10.1111/j.1600-0897.2009.00760.x
Language:
English
Publisher:
Wiley
Publication Date:
2009
detail.hit.zdb_id:
2024667-5
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