In:
Clinical and Developmental Immunology, Hindawi Limited, Vol. 2009 ( 2009), p. 1-8
Abstract:
Background . Vitamin D has been reported to regulate innate immunity by controlling the expression of antimicrobial peptides (AMPs). Objective . We investigated the effect of calcipotriol on the expression of AMPs in human cultured keratinocytes. Methods . Keratinocytes were treated with lipopolysaccharide (LPS), TNF- α , Calcipotriol and irradiated with UVB, cultured, and harvested. To assess the expression of human beta defensin-2 and LL-37 in the control group, not exposed to any stimulants, the experimental group was treated with LPS, TNF- α , or UVB, and another group was treated again with calcipotriol; reverse transcriptase-polymerase chain reaction, Western blotting, and immunohistochemical staining were performed. Results . In the experimental group treated with LPS, UVB irradiation, and TNF- α , the expression of β -defensin and LL-37 was increased more than in the control group and then decreased in the experimental group treated with calcipotriol. Conclusions . Calcipotriol suppressed HBD-2 and LL-37, which were stimulated by UVB, LPS, and TNF- α .
Type of Medium:
Online Resource
ISSN:
1740-2522
,
1740-2530
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2009
detail.hit.zdb_id:
2817541-4
detail.hit.zdb_id:
2119272-8
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