In:
Journal of Veterinary Diagnostic Investigation, SAGE Publications, Vol. 13, No. 6 ( 2001-11), p. 521-523
Abstract:
This report describes the successful adaptation of the Israeli isolate of Ehrlichia canis on a continuous mouse macrophage cell line (J774.A1). Successful infection of the J774.A1 cells was first judged by the direct immunofluorescence antibody test using an anti- E. canis-IgG:FITC conjugate. A particular property of infected J774.A1 cells was the ability to reestablish after harvesting of the monolayer by scaping. Infected cells were used as antigen for immunofluorescence antibody tests (IFA), and the results compared well with those of DH82 cells. It was concluded that the J774.A1 continuous cell line could serve as an alternate propagation cell line for E. canis organisms.
Type of Medium:
Online Resource
ISSN:
1040-6387
,
1943-4936
DOI:
10.1177/104063870101300612
Language:
English
Publisher:
SAGE Publications
Publication Date:
2001
detail.hit.zdb_id:
2265211-5
SSG:
22
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