In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 73, No. 8_Supplement ( 2013-04-15), p. 3699-3699
Abstract:
Our recent study demonstrated the skin cancer chemoprevention potential of commonly used benzophenone sunscreen agents using the Epstein-Barr virus early antigen in vitro assay (Int. J. Cosmet. Sci. 2012: doi:10.1111/ics.12015). We now report the in vivo anti-tumor activity of two of the benzophenone sunscreens tested positive in the above in vitro assay, octabenzone (UV-1) and dioxybenzone (UV-2), in the two-stage mouse skin carcinogenesis test model utilizing (±)-(E)-4-methyl-2-[-(E)-hydroxyamino]-5-nitro-6-methoxy-3-hexanamide (NOR-1) as inducer and 12-O-tetradecanoyl-phorbol-13-acetate (TPA) as promoter. Studies were conducted in pathogen-free, female hairless mice of HOS-HR-1 strain, 15 animals per control and test groups. Tumor was induced by a single dose of NOR-1 (390 nmol in 100 μl of acetone). One week later, TPA (1.7 nmol in 100 μl of acetone) was applied to skin twice weekly for 20 weeks as a tumor promoter. The test compounds, 0.0025% of UV-1 or UV-2 were administered to the animals through drinking water ad libitum, starting 1 week prior to tumor initiation and stopping 1 week after tumor initiation. All animals were examined weekly for the development of skin papillomas and the following were recorded: the week papillomas appeared, number of mice bearing papillomas and number of papil lomas per mouse. In both UV-1 and UV-2 treated mice, 2-week delay in tumor appearance and significant inhibition of tumor incidence (50% and 60%, respectively) and burden (papilloma inhibition/mouse, 50% and 70%, respectively) were observed when compared to the positive control group. Also, treatment with both benzophenone sunscreens produced marked reduction in the average number of tumors formed per mouse (5 and 3 papillomas/mouse for UV-1 and UV-2, respectively compared to 10 papillomas in positive control group) at the end of the 20-week experimental period. These in vivo results in mice further affirm the skin cancer chemoprevention potential of benzophenone sunscreen use in humans. Citation Format: Govind J. Kapadia, G Subba Rao, Midori Takasaki, Akira Iida, Nobutaka Suzuki, Takao Konoshima, Harukuni Tokuda. Chemoprevention of skin cancer by benzophenone sunscreen agents in mice. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3699. doi:10.1158/1538-7445.AM2013-3699
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2013-3699
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2013
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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