In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 72, No. 8_Supplement ( 2012-04-15), p. 4012-4012
Abstract:
Tumor cells take up more glucose than normal cells and convert large amounts of glucose to lactate even in the presence of oxygen. This phenomenon is known as aerobic glycolysis, and is important for cell growth and metastasis. PKM2 is an isoenzyme of the glycolytic enzyme pyruvate kinase, and promotes aerobic glycolysis. Here, we describe an important role for PKM2 in regulating the survival and migration of gastric cancer cells. Using qRT-PCR and tissue microarray, we showed that PKM2 was overexpressed in gastric tumor tissues compared to normal tissues. Furthermore, PKM2 expression levels were inversely correlated with gastric cancer patient survival. Using shRNA-mediated knockdown of PKM2, we showed that PKM2 regulated apoptosis-related gene, Bcl-xL, at transcriptional levels, and affected cell survival. PKM2 also regulated migration-related gene, MMP-9, at transcriptional levels, and affected cell migration. The effect of PKM2 on cell death and migration was mediated by the p65 stability resulting in binding activity on its target gene promoter. These results provide new insight that PKM2 is an up-stream molecule regulating p65 function and that PKM2 may be a useful target for gastric cancer therapy. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4012. doi:1538-7445.AM2012-4012
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2012-4012
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2012
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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