In:
Microcirculation, Wiley, Vol. 25, No. 2 ( 2018-02)
Abstract:
The aim of this study was to examine the effect of TXL , a Chinese medicine prescription, on cerebral microcirculatory disturbances after p MCAO in mice using TPLSM and further explore the underlying mechanisms. Methods Adlut male C 57 BL /6 J mice were subjected to p MCAO and orally administered with TXL (3.0, 1.5 and 0.75 g/kg/d) at 1, 3, and 21 hours after p MCAO . The following parameters were examined at 6 and 24 hours after p MCAO : neurological deficits, infarct volume, BBB permeability, cerebral microvessel structure, brain microcirculation ( TPLSM imaging), vasoactive factors, and adhesion molecules. Results TXL improved neurological deficits, reduced infarct volume, attenuated BBB disruption, protected cerebral microvessel structure, increased cerebral capillary flow velocity and volume flux, and inhibited leukocyte–endothelial cell interactions at 6 or 24 hours after p MCAO . The therapeutic efficacy was exerted in a dose‐dependent manner. Further study revealed that TXL (high dose) regulated the expression of PGI 2, TXA 2, and ET ‐1, and suppressed ICAM ‐1 and P‐selectin. Conclusions TXL alleviates cerebral microcirculatory disturbances against ischemic injury by modulating endothelial function and inhibiting leukocyte–endothelial cell interactions. These effects are associated with regulating the expression of PGI 2, TXA 2, and ET ‐1, and suppressing ICAM ‐1 and P‐selectin expression.
Type of Medium:
Online Resource
ISSN:
1073-9688
,
1549-8719
DOI:
10.1111/micc.2018.25.issue-2
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2008083-9
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