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  • Springer Science and Business Media LLC  (9)
  • Kang, Minhee  (9)
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  • Springer Science and Business Media LLC  (9)
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  • 1
    Online Resource
    Online Resource
    Loop-mediated isothermal amplification assay for screening congenital cytomegalovirus infection in newborns
    Springer Science and Business Media LLC ; 2023
    In:  Applied Microbiology and Biotechnology
    Details
    In: Applied Microbiology and Biotechnology, Springer Science and Business Media LLC
    Abstract: Congenital cytomegalovirus (CMV) infection is a common cause of sensorineural hearing loss and neurodevelopmental impairment in newborns. However, congenital CMV infection cannot be diagnosed using samples collected more than 3 weeks after birth because testing after this time cannot distinguish between congenital infection and postnatal infection. Herein, we developed a robust loop-mediated isothermal amplification (LAMP) assay for the large-scale screening of newborns for congenital CMV infection. In contrast to conventional quantitative polymerase chain reaction (qPCR), which detects CMV within a dynamic range of 1.0 × 10 6 to 1.0 × 10 2 copies/μL, our quantitative LAMP assay (qLAMP) detects CMV within a dynamic range of 1.1 × 10 8 to 1.1 × 10 3 copies/μL. Moreover, the turnaround time for obtaining results following DNA extraction is 90 min in qPCR but only 15 min in qLamp. The colorimetric LAMP assay can also detect CMV down to 1.1 × 10 3 copies/μL within 30 min, irrespective of the type of heat source. Our LAMP assay can be utilized in central laboratories as an alternative to conventional qPCR for quantitative CMV detection, or for point-of-care testing in low-resource environments, such as developing countries, via colorimetric naked-eye detection. Key points • LAMP assay enables large-scale screening of newborns for congenital CMV infection. • LAMP allows colorimetric or quantitative detection of congenital CMV infection. • LAMP assay can be used as a point-of-care testing tool in low-resource environments.
    Type of Medium: Online Resource
    ISSN: 0175-7598 , 1432-0614
    URL: Article
    DOI: 10.1007/s00253-023-12771-2
    RVK:
    WA 15000
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 1464336-4
    SSG: 12
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    Online Resource
  • 2
    Online Resource
    Online Resource
    A Simple and Multiplex Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of SARS-CoV
    Springer Science and Business Media LLC ; 2019
    In:  BioChip Journal Vol. 13, No. 4 ( 2019-12), p. 341-351
    Details
    In: BioChip Journal, Springer Science and Business Media LLC, Vol. 13, No. 4 ( 2019-12), p. 341-351
    Type of Medium: Online Resource
    ISSN: 1976-0280 , 2092-7843
    URL: Article
    DOI: 10.1007/s13206-019-3404-3
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2019
    detail.hit.zdb_id: 2513796-7
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    Online Resource
  • 3
    Online Resource
    Online Resource
    Optimization of extraction-free protocols for SARS-CoV-2 detection using a commercial rRT-PCR assay
    Springer Science and Business Media LLC ; 2023
    In:  Scientific Reports Vol. 13, No. 1 ( 2023-11-21)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2023-11-21)
    Abstract: In the ongoing global fight against coronavirus disease 2019 (COVID-19), the sample preparation process for real-time reverse transcription polymerase chain reaction (rRT-PCR) faces challenges due to time-consuming steps, labor-intensive procedures, contamination risks, resource demands, and environmental implications. However, optimized strategies for sample preparation have been poorly investigated, and the combination of RNase inhibitors and Proteinase K has been rarely considered. Hence, we investigated combinations of several extraction-free protocols incorporating heat treatment, sample dilution, and Proteinase K and RNase inhibitors, and validated the effectiveness using 120 SARS-CoV-2 positive and 62 negative clinical samples. Combining sample dilution and heat treatment with Proteinase K and RNase inhibitors addition exhibited the highest sensitivity (84.26%) with a mean increase in cycle threshold (Ct) value of + 3.8. Meanwhile, combined sample dilution and heat treatment exhibited a sensitivity of 79.63%, accounting for a 38% increase compared to heat treatment alone. Our findings highlight that the incorporation of Proteinase K and RNase inhibitors with sample dilution and heat treatment contributed only marginally to the improvement without yielding statistically significant differences. Sample dilution significantly impacts SARS-CoV-2 detection, and sample conditions play a crucial role in the efficiency of extraction-free methods. Our findings may provide insights for streamlining diagnostic testing, enhancing its accessibility, cost-effectiveness, and sustainability.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/s41598-023-47645-0
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2615211-3
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    Online Resource
  • 4
    Online Resource
    Online Resource
    Rapid species identification of pathogenic bacteria from a minute quantity exploiting three-dimensional quantitative phase imaging and artificial neural network
    Springer Science and Business Media LLC ; 2022
    In:  Light: Science & Applications Vol. 11, No. 1 ( 2022-06-23)
    Details
    In: Light: Science & Applications, Springer Science and Business Media LLC, Vol. 11, No. 1 ( 2022-06-23)
    Abstract: The healthcare industry is in dire need of rapid microbial identification techniques for treating microbial infections. Microbial infections are a major healthcare issue worldwide, as these widespread diseases often develop into deadly symptoms. While studies have shown that an early appropriate antibiotic treatment significantly reduces the mortality of an infection, this effective treatment is difficult to practice. The main obstacle to early appropriate antibiotic treatments is the long turnaround time of the routine microbial identification, which includes time-consuming sample growth. Here, we propose a microscopy-based framework that identifies the pathogen from single to few cells. Our framework obtains and exploits the morphology of the limited sample by incorporating three-dimensional quantitative phase imaging and an artificial neural network. We demonstrate the identification of 19 bacterial species that cause bloodstream infections, achieving an accuracy of 82.5% from an individual bacterial cell or cluster. This performance, comparable to that of the gold standard mass spectroscopy under a sufficient amount of sample, underpins the effectiveness of our framework in clinical applications. Furthermore, our accuracy increases with multiple measurements, reaching 99.9% with seven different measurements of cells or clusters. We believe that our framework can serve as a beneficial advisory tool for clinicians during the initial treatment of infections.
    Type of Medium: Online Resource
    ISSN: 2047-7538
    URL: Article
    DOI: 10.1038/s41377-022-00881-x
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2662628-7
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  • 5
    Online Resource
    Online Resource
    Gold nanostar-modified electrochemical sensor for highly sensitive renin quantification as a marker of tissue-perfusion
    Springer Science and Business Media LLC ; 2023
    In:  MRS Communications
    Details
    In: MRS Communications, Springer Science and Business Media LLC
    Type of Medium: Online Resource
    ISSN: 2159-6867
    URL: Article
    DOI: 10.1557/s43579-023-00414-6
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2645443-9
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  • 6
    Online Resource
    Online Resource
    Comparison and Analysis of Polymer-Functionalized Carbon Nanotubes for Enhancement of the Quantitative Detection of Procalcitonin Levels in Human Plasma
    Springer Science and Business Media LLC ; 2023
    In:  BioChip Journal Vol. 17, No. 2 ( 2023-06), p. 274-283
    Details
    In: BioChip Journal, Springer Science and Business Media LLC, Vol. 17, No. 2 ( 2023-06), p. 274-283
    Type of Medium: Online Resource
    ISSN: 1976-0280 , 2092-7843
    URL: Article
    DOI: 10.1007/s13206-023-00102-6
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2513796-7
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  • 7
    Online Resource
    Online Resource
    The long-lasting post-stimulation inhibitory effects of bladder activity induced by posterior tibial nerve stimulation in unanesthetized rats
    Springer Science and Business Media LLC ; 2020
    In:  Scientific Reports Vol. 10, No. 1 ( 2020-11-16)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 10, No. 1 ( 2020-11-16)
    Abstract: Tibial nerve stimulation (TNS) is one of the neuromodulation methods used to treat an overactive bladder (OAB). However, the treatment mechanism is not accurately understood owing to significant differences in the results obtained from animal and clinical studies. Thus, this study was aimed to confirm the response of bladder activity to the different stimulation frequencies and to observe the duration of prolonged post-stimulation inhibitory effects following TNS. This study used unanesthetized rats to provide a closer approximation of the clinical setting and evaluated the changes in bladder activity in response to 30 min of TNS at different frequencies. Moreover, we observed the long-term changes of post-stimulation inhibitory effects. Our results showed that bladder response was immediately inhibited after 30 min of 10 Hz TNS, whereas it was excited at 50 Hz TNS. We also used the implantable stimulator to observe a change in duration of the prolonged post-stimulation inhibitory effects of the TNS and found large discrepancies in the time that the inhibitory effect lasted after stimulation between individual animals. This study provides important evidence that can be used to understand the neurophysiological mechanisms underlying the bladder inhibitory response induced by TNS as well as the long-lasting prolonged post-stimulation effect.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/s41598-020-76987-2
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2615211-3
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  • 8
    Online Resource
    Online Resource
    Backbone assignment and inhibitor binding studies of IL-33 mutants by NMR spectroscopy
    Springer Science and Business Media LLC ; 2023
    In:  Journal of Analytical Science and Technology Vol. 14, No. 1 ( 2023-07-24)
    Details
    In: Journal of Analytical Science and Technology, Springer Science and Business Media LLC, Vol. 14, No. 1 ( 2023-07-24)
    Abstract: Interleukin-33 (IL-33) is an IL-1 family protein that induces a type-2 immune response. IL-33 is constitutively expressed in epithelial cells and released in response to the cell damage or stimulation by an allergen. The secreted protein is activated when the N-terminal domain is cleaved by a protease, and the active form signals downstream immune cells, such as eosinophils, by binding to the heterodimeric ST2:IL-1RAcP receptor complex on the cell surface. The binding stimulates an inflammatory response, and the abnormal inflammatory response can cause allergic diseases such as atopic dermatitis and asthma. Inhibition of the interaction between IL-33 and ST2 is an attractive target to control the inflammatory disease at the upstream of the signaling. However, discovering the chemical moieties that bind to the protein–protein interaction interface is a challenging task due to the relatively wide and shallow binding pocket compared to the enzyme’s active site. For the IL-33-specific binder discovery, a series of IL-33 mutants were designed, and an electrophile chemical library was screened. Herein, we described the backbone 1 H, 15 N, and 13 C resonance assignments of three IL-33 (117–270) mutants. Based on the assignments, the binding site of a selected compound by this approach was determined by 2D NMR. These results provide valuable information for further studies in drug discovery targeting the IL-33 and ST2 interaction.
    Type of Medium: Online Resource
    ISSN: 2093-3371
    URL: Article
    DOI: 10.1186/s40543-023-00392-3
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2632347-3
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  • 9
    Online Resource
    Online Resource
    Multiplex Molecular Point-of-Care Test for Syndromic Infectious Diseases
    Springer Science and Business Media LLC ; 2021
    In:  BioChip Journal Vol. 15, No. 1 ( 2021-03), p. 14-22
    Details
    In: BioChip Journal, Springer Science and Business Media LLC, Vol. 15, No. 1 ( 2021-03), p. 14-22
    Abstract: Point-of-care (POC) molecular diagnostics for clinical microbiology and virology has primarily focused on the detection of a single pathogen. More recently, it has transitioned into a comprehensive syndromic approach that employs multiplex capabilities, including the simultaneous detection of two or more pathogens. Multiplex POC tests provide higher accuracy to for actionable decisionmaking in critical care, which leads to pathogen-specific treatment and standardized usages of antibiotics that help prevent unnecessary processes. In addition, these tests can be simple enough to operate at the primary care level and in remote settings where there is no laboratory infrastructure. This review focuses on state-of-the-art multiplexed molecular point-of-care tests (POCT) for infectious diseases and efforts to overcome their limitations, especially related to inadequate throughput for the identification of syndromic diseases. We also discuss promising and imperative clinical POC approaches, as well as the possible hurdles of their practical applications as front-line diagnostic tests.
    Type of Medium: Online Resource
    ISSN: 1976-0280 , 2092-7843
    URL: Article
    DOI: 10.1007/s13206-021-00004-5
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2513796-7
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