In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 10 ( 2022-10-3), p. e0275476-
Abstract:
DEK has a short isoform (DEK isoform-2; DEK2) that lacks amino acid residues between 49–82. The full-length DEK (DEK isoform-1; DEK1) is ubiquitously expressed and plays a role in different cellular processes but whether DEK2 is involved in these processes remains elusive. We stably overexpressed DEK2 in human bone marrow stromal cell line HS-27A, in which endogenous DEKs were intact or suppressed via short hairpin RNA (sh-RNA). We have found that contrary to ectopic DEK1, DEK2 locates in the nucleus and nucleolus, causes persistent γH2AX signal upon doxorubicin treatment, and couldn’t functionally compensate for the loss of DEK1. In addition, DEK2 overexpressing cells were more sensitive to doxorubicin than DEK1-cells. Expressions of DEK1 and DEK2 in cell lines and primary tumors exhibit tissue specificity. DEK1 is upregulated in cancers of the colon, liver, and lung compared to normal tissues while both DEK1 and DEK2 are downregulated in subsets of kidney, prostate, and thyroid carcinomas. Interestingly, only DEK2 was downregulated in a subset of breast tumors suggesting that DEK2 can be modulated differently than DEK1 in specific cancers. In summary, our findings show distinct expression patterns and subcellular location and suggest non-overlapping functions between the two DEK isoforms.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0275476
DOI:
10.1371/journal.pone.0275476.g001
DOI:
10.1371/journal.pone.0275476.g002
DOI:
10.1371/journal.pone.0275476.g003
DOI:
10.1371/journal.pone.0275476.g004
DOI:
10.1371/journal.pone.0275476.g005
DOI:
10.1371/journal.pone.0275476.g006
DOI:
10.1371/journal.pone.0275476.t001
DOI:
10.1371/journal.pone.0275476.t002
DOI:
10.1371/journal.pone.0275476.s001
DOI:
10.1371/journal.pone.0275476.s002
DOI:
10.1371/journal.pone.0275476.s003
DOI:
10.1371/journal.pone.0275476.s004
DOI:
10.1371/journal.pone.0275476.s005
DOI:
10.1371/journal.pone.0275476.s006
DOI:
10.1371/journal.pone.0275476.r001
DOI:
10.1371/journal.pone.0275476.r002
DOI:
10.1371/journal.pone.0275476.r003
DOI:
10.1371/journal.pone.0275476.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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