In:
Current Protocols in Toxicology, Wiley, Vol. 80, No. 1 ( 2019-06)
Abstract:
Using galactose instead of glucose in the culture medium of hepatoma cell lines, such as HepG2 cells, has been utilized for a decade to unmask the mitochondrial liability of chemical compounds. A modified glucose‐galactose assay on HepG2 cells, reducing the experimental period for screening of mitochondrial toxicity to 2 to 4 hr, has been previously reported. HepaRG cells are one of the few cell lines that retain some of the important characteristics of human hepatocytes, offering advantages of working with a cell line, therefore, are considered an alternative for HepG2 cells in drug toxicity screening. A method is described here using HepaRG cells in an acute metabolic switch assay utilizing specific glucose/galactose media, a combined ATP‐protein‐LDH assay measuring three endpoints from one 96‐well plate, and a criteria to label a compound as a mitochondrial toxin. © 2019 by John Wiley & Sons, Inc.
Type of Medium:
Online Resource
ISSN:
1934-9254
,
1934-9262
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
2179079-6
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