In:
International Archives of Allergy and Immunology, S. Karger AG, Vol. 59, No. 4 ( 1979), p. 408-419
Abstract:
Under monitoring the antigenic activity by radioimmunoassay (RIA), one of the major sea-squirt antigens was isolated by gel chromatography after treatment of the extract from internal organs of sea-squirt with QAE-Sephadex. This antigen was fairly homogeneous as judged from its elution profile in chromatography on a column of Ultrogel AcA 44 with respect not only of the antigenic activity but also to UV absorption. Furthermore, the antigen inhibited the binding of the 〈 sup 〉 125 〈 /sup 〉 I-labeled tracer antigen to rabbit anti-sea-squirt antibody in a similar mode to the case of Ei-2, one of the sea-squirt antigens previously prepared, but its specific activity was as high as 2.33 times that of Ei-2. Accordingly, this isolated antigen was corresponded to the major antigenic substance in Ei-2 and referred to as 〈 i 〉 Ei-M 〈 /i 〉 . In addition, the other antigen of 〈 i 〉 Gi-x 〈 /i 〉 was also isolated by chromatography on a column of Sepharose 6B as the major antigenic substance in Gi-2, the other one of the previous antigens. Gi-x was apparently different from Ei-M in the mode of inhibition of the binding of the tracer antigen to the antibody, although its antigenic activity against the asthmatic patients 〈 i 〉 in vivo 〈 /i 〉 was as high as that of Ei-M. Thus, it was considered that sea-squirt contained at least two types of antigens of Ei-M and Gi-x.
Type of Medium:
Online Resource
ISSN:
1018-2438
,
1423-0097
Language:
English
Publisher:
S. Karger AG
Publication Date:
1979
detail.hit.zdb_id:
1482722-0
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