In:
American Journal of Physiology-Lung Cellular and Molecular Physiology, American Physiological Society, Vol. 273, No. 4 ( 1997-10-01), p. L775-L781
Abstract:
Muscarinic receptor stimulation increases Ca 2+ sensitivity, i.e., the amount of force produced at a constant submaximal cytosolic Ca 2+ concentration ([Ca 2+ ] i ), in permeabilized smooth muscle preparations. It is controversial whether this increase in Ca 2+ sensitivity is in part mediated by protein kinase C (PKC). With the use of a β-escin permeabilized canine tracheal smooth muscle (CTSM) preparation, the effect of four putative PKC inhibitors {calphostin C, chelerythrine chloride, a pseudosubstrate inhibitor for PKC [PKC peptide-(19—31)], and staurosporine} on Ca 2+ sensitization induced by acetylcholine (ACh) plus GTP was determined. Preincubation with each of the inhibitors did not affect subsequent Ca 2+ sensitization induced by muscarinic receptor stimulation in the presence of a constant submaximal [Ca 2+ ] i , neither did any of these compounds reverse the increase in Ca 2+ sensitivity induced by ACh plus GTP. Administration of a 1,2-diacylglycerol analog, 1-oleoyl-2-acetyl- sn-glycerol, did not induce Ca 2+ sensitization at a constant submaximal [Ca 2+ ] i . Thus we found no evidence that PKC mediates increases in Ca 2+ sensitivity produced by muscarinic receptor stimulation in permeabilized CTSM.
Type of Medium:
Online Resource
ISSN:
1040-0605
,
1522-1504
DOI:
10.1152/ajplung.1997.273.4.L775
Language:
English
Publisher:
American Physiological Society
Publication Date:
1997
detail.hit.zdb_id:
1477300-4
SSG:
12
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