ISSN:
1432-0983
Schlagwort(e):
DNA repair
;
RAD6 gene
;
RAD52 gene
;
UV mutagenesis
;
Mating type
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract The RAD6 gene of Saccharomyces cerevisiae is required for post-replication repair of UV-damaged DNA, UV mutagenesis, and sporulation. Here, we show that the radiation sensitivity of a MAT a rad6Δ strain can be suppressed by the MATα2 gene carried on a multicopy plasmid. The a1-α2 suppression is specific to the RAD6 pathway, as mutations in genes required for nucleotide excision repair or for recombinational repair do not show such mating-type suppression. The a1-α2 suppression of the rad6Δ mutation requires the activity of the RAD52 group of genes, suggesting that suppression occurs by channelling of post-replication gaps present in the rad6Δ mutant into the RAD52 recombinational repair pathway. The a1-α2 repressor could mediate this suppression via an enhancement in the expression, or the activity, of recombination genes.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00311876
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