In:
Molecular Microbiology, Wiley, Vol. 66, No. 2 ( 2007-10), p. 371-382
Abstract:
A grobacterium t umefaciens‐ m ediated t ransformation (ATMT) has become a prevalent tool for functional genomics of fungi, but our understanding of T‐DNA integration into the fungal genome remains limited relative to that in plants. Using a model plant‐pathogenic fungus, Magnaporthe oryzae , here we report the most comprehensive analysis of T‐DNA integration events in fungi and the development of an informatics infrastructure, termed a T ‐DNA a nalysis p latform (TAP). We identified a total of 1110 T ‐DNA‐ t agged l ocations (TTLs) and processed the resulting data via TAP. Analysis of the TTLs showed that T‐DNA integration was biased among chromosomes and preferred the promoter region of genes. In addition, irregular patterns of T‐DNA integration, such as chromosomal rearrangement and readthrough of plasmid vectors, were also observed, showing that T‐DNA integration patterns into the fungal genome are as diverse as those of their plant counterparts. However, overall the observed junction structures between T‐DNA borders and flanking genomic DNA sequences revealed that T‐DNA integration into the fungal genome was more canonical than those observed in plants. Our results support the potential of ATMT as a tool for functional genomics of fungi and show that the TAP is an effective informatics platform for handling data from large‐scale insertional mutagenesis.
Type of Medium:
Online Resource
ISSN:
0950-382X
,
1365-2958
DOI:
10.1111/mmi.2007.66.issue-2
DOI:
10.1111/j.1365-2958.2007.05918.x
Language:
English
Publisher:
Wiley
Publication Date:
2007
detail.hit.zdb_id:
1501537-3
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