In:
AIDS Research and Therapy, Springer Science and Business Media LLC, Vol. 7, No. 1 ( 2010-12)
Abstract:
HIV-1 in genital secretions may be opsonized by several molecules including complement components. Opsonized HIV-1 by complement enhances the infection of various mucosal target cells, such as dendritic cells (DC) and epithelial cells. Results We herein evaluated the effect of HIV-1 complement opsonization on microbicide candidates' activity, by using three in vitro mucosal models: CCR5-tropic HIV-1 JR-CSF transcytosis through epithelial cells, HIV-1 JR-CSF attachment on immature monocyte-derived dendritic cells (iMDDC), and infectivity of iMDDC by CCR5-tropic HIV-1 BaL and CXCR4-tropic HIV-1 NDK . A panel of 10 microbicide candidates [T20, CADA, lectines HHA & GNA, PVAS, human lactoferrin, and monoclonal antibodies IgG1B12, 12G5, 2G12 and 2F5], were investigated using cell-free unopsonized or opsonized HIV-1 by complements. Only HHA and PVAS were able to inhibit HIV trancytosis. Upon opsonization, transcytosis was affected only by HHA, HIV-1 adsorption on iMDDC by four molecules (lactoferr in, IgG1B12, IgG2G5, IgG2G12), and replication in iMDDC of HIV-1 BaL by five molecules (lactoferrin, CADA, T20, IgG1B12, IgG2F5) and of HIV-1 NDK by two molecules (lactoferrin, IgG12G5). Conclusion These observations demonstrate that HIV-1 opsonization by complements may modulate in vitro the efficiency of candidate microbicides to inhibit HIV-1 infection of mucosal target cells, as well as its crossing through mucosa.
Type of Medium:
Online Resource
ISSN:
1742-6405
DOI:
10.1186/1742-6405-7-16
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2010
detail.hit.zdb_id:
2173450-1
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