In:
Thrombosis and Haemostasis, Georg Thieme Verlag KG, Vol. 42, No. 04 ( 1979), p. 1153-1158
Abstract:
Fibrin polymers formed from fibrinogen with thrombin in the presence of EDTA were suspended in a medium containing glucose, arabic gum and imidazole-HCl buffer and were sonicated at 20 kHz for 20 min to make a suspension containing fibrin particles of small size. The fibrin suspension was used as a substrate of plasmin for determining the enzymic activity of plasmin and plasminogen activated with urokinase. The kinetic study on the reaction of the fibrin particles with plasmin in the presence and the absence of fibrinogen revealed that Km value of fibrin for plasmin is 4.2 × 10-7 M and the Ki value of fibrinogen is 1.2 × 10-5 M.
Type of Medium:
Online Resource
ISSN:
0340-6245
,
2567-689X
DOI:
10.1055/s-0038-1657010
Language:
English
Publisher:
Georg Thieme Verlag KG
Publication Date:
1979
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