In:
Transfusion, Wiley, Vol. 47, No. 7 ( 2007-07), p. 1254-1262
Abstract:
BACKGROUND: Second‐generation platelet (PLT) substitutes for treatment of bleeding were studied and the focus was on a dodecapeptide, HHLGGAKQAGDV (H12), which is a fibrinogen γ‐chain carboxy‐terminal sequence (γ 400‐411) and exists only in a fibrinogen domain. STUDY DESIGN AND METHODS: H12 was conjugated to the surface of polymerized albumin particles (polyAlb) modified with polyethylene glycol (PEG) chains to produce biocompatible particles (H12‐PEG‐polyAlb) that had prolonged blood circulation t ½ and were more stable in vitro and in vivo compared with H12‐polyAlb (not modified with PEG). H12‐PEG‐polyAlb was administered intravenously into thrombocytopenic rats and the t ½ of the particles and the tail bleeding time were measured to evaluate the prolongation in the hemostatic effect. RESULTS: H12‐PEG‐polyAlb particles modified with PEG prolonged the t ½ and maintained specific binding ability to activated PLTs. The particles dose dependently shortened the tail bleeding time of thrombocytopenic rats 6 hours after injection. CONCLUSION: H12‐PEG‐polyAlb may be a suitable candidate for treatment of bleeding into thrombocytopenic patients as an alternative to PLT concentrate transfusion.
Type of Medium:
Online Resource
ISSN:
0041-1132
,
1537-2995
DOI:
10.1111/trf.2007.47.issue-7
DOI:
10.1111/j.1537-2995.2007.01265.x
Language:
English
Publisher:
Wiley
Publication Date:
2007
detail.hit.zdb_id:
2018415-3
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