In:
Annals of the Entomological Society of America, Oxford University Press (OUP), Vol. 105, No. 6 ( 2012-11-01), p. 890-895
Abstract:
A novel technique of insect ecdysone analysis was established by capillary zone electrophoresis using 20-hydroxy ecdysone (purity ≥93%) as a standard sample. It showed that 20-hydroxy ecdysone and other trace impurities were completely separated within 5 min with an electrolyte containing 10 mmol/L borate, at pH 9.18, 20 kV applied voltage and 0.5 psi × 5 s injected volume. Under this optimal condition, effects of azadirachtin on ecdysone in the hemolymph of Ostrinia furnacalis (Guenee) were investigated. The results showed that the peak values of 20-hydroxy ecdysone were strongly suppressed by azadirachtin and the titers remained at a lower level than those in the control samples. When compared with high-performance liquid chromatography, the primary advantages of capillary zone electrophoresis include a much shorter analysis time and detection limit and the elimination of complex pretreatment of samples.
Type of Medium:
Online Resource
ISSN:
1938-2901
,
0013-8746
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2012
detail.hit.zdb_id:
2175906-6
detail.hit.zdb_id:
2028805-0
SSG:
12
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